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AB309280

Human BFL-1/GRS ELISA Kit

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Human BFL-1/GRS ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BFL-1/GRS with a sensitivity of 0.049 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BCL2L5, BFL1, GRS, HBPA1, BCL2A1, Bcl-2-related protein A1, Bcl-2-like protein 5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS, Bcl2-L-5

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Sandwich ELISA - Human BFL-1/GRS ELISA Kit (AB309280)
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Supplier Data

Sandwich ELISA - Human BFL-1/GRS ELISA Kit (AB309280)

Interpolated concentrations of human BFL-1/GRS in U-937 cell extract

Interpolated concentration of native BFL-1/GRS was measured in duplicate at different sample concentrations. The undiluted sample is 250 µg/mL U-937 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human BFL-1/GRS ELISA Kit (AB309280)
  • sELISA

Supplier Data

Sandwich ELISA - Human BFL-1/GRS ELISA Kit (AB309280)

Example of human BFL-1/GRS standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.049 ng/mL

Range

0.094 - 6 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB45413

Anti-BFL-1/GRS antibody

Immunocytochemistry - Anti-BFL-1/GRS antibody (AB45413)

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Proteins & Peptides

AB126683

Recombinant Human BFL-1/GRS protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human BFL-1/GRS protein (His tag N-Terminus) (AB126683)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human BFL-1/GRS SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BFL-1/GRS protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human BFL-1/GRS with 0.049 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "90 - 95 %", "average": "= 92" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309280-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB309280-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB309280-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309280-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309280-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309280-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309280-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309280-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309280-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BFL-1/GRS Detector Antibody", "number":"AB309280-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human BFL-1/GRS Lyophilized Recombinant Protein", "number":"AB309280-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BFL-1/GRS Capture Antibody", "number":"AB309280-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity). Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11 (By similarity).
See full target information BCL2A1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com