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AB313512

Human BLMH ELISA Kit

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Human BLMH ELISA Kit is a Sandwich ELISA for the measurement of Human BLMH in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH

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Sandwich ELISA - Human BLMH ELISA Kit (AB313512)
  • sELISA

Supplier Data

Sandwich ELISA - Human BLMH ELISA Kit (AB313512)

Example of standard curve using ab313512. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.085 ng/mL

Range

0.085 - 20 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB204584

Anti-BLMH antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLMH antibody (AB204584)

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Proteins & Peptides

AB101205

Recombinant Human BLMH protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human BLMH protein (His tag N-Terminus) (AB101205)

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Cell Lines & Lysates

AB265361

Human BLMH knockout HeLa cell line

Sanger Sequencing - Human BLMH knockout HeLa cell line (AB265361)

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Cell Lines & Lysates

AB258792

Human BLMH knockout HeLa cell lysate

Sanger Sequencing - Human BLMH knockout HeLa cell lysate (AB258792)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human BLMH (Bleomycin Hydrolase) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BLMH in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human BLMH coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Azurocidin, C1q R1, C1qTNF1, C2, Cathepsin A, Cathepsin D, Cathepsin X/Z/P, Aminopeptidase N, CD35, Contactin-4, CPA1, CPM, DNER, Endorepellin, Fetuin B, Hepassocin, LEKTI, MAGP-2/MFAP5, MCAM, MMR, Neuropilin-1, PAM, Park7, PCSK9, PLUNC, PRCP, PRTN3, P-Selectin, PSP94, S100A6, SAA, SLPI, SPARCL1, Tenascin C, Thioredoxin-1, TIM-4, Trypsin Pan, Thrombospondin-4, VSIG4.

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Recovery

[ { "sample": "Cell culture media", "range": "80 - 100 %", "average": "= 92.08" }, { "sample": "Plasma", "range": "85 - 105 %", "average": "= 95.75" }, { "sample": "Serum", "range": "97 - 104 %", "average": "= 101.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313512-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human BLMH Protein Standard", "number":"AB313512-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human BLMH Detection Antibody", "number":"AB313512-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313512-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313512-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB313512-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human BLMH Antibody-coated ELISA Plate", "number":"AB313512-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313512-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313512-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
See full target information BLMH
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com