Human BLMH ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human BLMH in Human in Cell culture supernatant, Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.085 - 20 ng/mL
- Sensitivity
- = 0.085 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
Alternative names
Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH
What's included?
Recommended products
Human BLMH ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human BLMH in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.085 - 20 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.085 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture media | Average % = 92.08 | Range 80 - 100 % |
Sample type Plasma | Average % = 95.75 | Range 85 - 105 % |
Sample type Serum | Average % = 101.7 | Range 97 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human BLMH (Bleomycin Hydrolase) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BLMH in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human BLMH coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Azurocidin, C1q R1, C1qTNF1, C2, Cathepsin A, Cathepsin D, Cathepsin X/Z/P, Aminopeptidase N, CD35, Contactin-4, CPA1, CPM, DNER, Endorepellin, Fetuin B, Hepassocin, LEKTI, MAGP-2/MFAP5, MCAM, MMR, Neuropilin-1, PAM, Park7, PCSK9, PLUNC, PRCP, PRTN3, P-Selectin, PSP94, S100A6, SAA, SLPI, SPARCL1, Tenascin C, Thioredoxin-1, TIM-4, Trypsin Pan, Thrombospondin-4, VSIG4.
Product promise
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Terms & Conditions.
1 product image
Sandwich ELISA - Human BLMH ELISA Kit (ab313512)
Example of standard curve using ab313512. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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