AB272210

Human BMI1 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human BMI1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BMI1 with a sensitivity of 34 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PCGF4, RNF51, BMI1, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51

4 Images

Sandwich ELISA - Human BMI1 ELISA Kit (AB272210)

sELISA

Supplier Data

Sandwich ELISA - Human BMI1 ELISA Kit (AB272210)

Species reactivity was determined by measuring 100 ug/mL extract of human HeLa, mouse NIH-3T3 and rat PC12 cell extracts and interpolating the protein concentrations from the Human standard curve.

The mean BMI1 concentration was determined to be 16.9 ng/mg in human HeLa extract, 12.8 ng/mg in mouse NIH-3T3 extract and 1.2 ng/mg in rat PC12 extract.

sELISA

Supplier Data

Sandwich ELISA - Human BMI1 ELISA Kit (AB272210)

Representative image of Human BMI1 ELISA Kit (ab272210)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human BMI1 ELISA Kit Capture Antibody

- 10X Human BMI1 Detector Antibody

- 10X Human BMI1 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human BMI1 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human BMI1 ELISA Kit (AB272210)

Interpolated concentrations of native BMI1 in human HeLa and K562 based on 300 μg/mL and 150 µg/mL extract loads, respectively.

The concentrations of BMI1 were measured in duplicate and interpolated from the BMI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMI1 concentration was determined to be 5,142 pg/mL in HeLa extract and 3,399 pg/mL in K562 extract.

sELISA

Supplier Data

Sandwich ELISA - Human BMI1 ELISA Kit (AB272210)

Example of human BMI1 standard curve in 1X Cell Extraction Buffer PTR.

The BMI1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 34 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human BMI1 ELISA kit (ab272210) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BMI1 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BMI1 with 34 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

BMI1 (Polycomb Complex Protein BMI-1), also known as Polycomb group RING Finger Protein 4, is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, BMI1 regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2. Human BMI1 amino acids sequence is 97% identical to mouse BMI1 and is 95% identical to rat BMI1.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.3" } ]

style

left-column

Recovery

[ { "sample": "Cell Lysate", "range": "112 - 115 %", "average": "= 114" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272210-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272210-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272210-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB272210-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272210-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272210-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272210-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB272210-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human BMI1 Lyophilized Recombinant Protein", "number":"AB272210-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BMI1 Capture Antibody", "number":"AB272210-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB272210-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272210-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BMI1 Detector Antibody", "number":"AB272210-CMP02", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bmi1 also known as B-cell-specific Moloney murine leukemia virus integration site 1 is a member of the Polycomb group of proteins. It has an approximate molecular mass of 37 kDa. Bmi1 is expressed in many tissues including the brain bone marrow and hematopoietic cells. It functions as a transcriptional repressor. Bmi1 plays an important role in chromatin remodeling influencing gene silencing and regulation of developmental processes. Bmi1 ELISA assays are commonly used for quantifying its levels in various research applications.

Biological function summary

The functions of Bmi1 extend into maintaining stem cell renewal and differentiation. It acts as part of the Polycomb repressive complex 1 (PRC1) partnering with other proteins to regulate gene expression by altering chromatin structure. Bmi1 protein regulates cell proliferation and inhibits senescence by repressing the INK4a/ARF locus which encodes tumor suppressor proteins like p16INK4a and p14ARF.

Pathways

Bmi1 plays significant roles in processes such as the DNA damage response and the self-renewal of hematopoietic stem cells. It is involved in the modulation of the Wnt and Notch signaling pathways. Bmi1 protein interacts closely with proteins such as CBX2 and RNF2 within these pathways affecting the transcriptional landscape that governs cell fate decisions.

Bmi1 overexpression links to cancer particularly breast cancer and leukemia. In MCF7 breast cancer cells alterations in Bmi1 activity contribute to oncogenesis and tumor progression. Its dysregulation can connect to Myc a well-known oncogenic protein. Additionally Bmi1's influence in Cas9-based gene editing models highlights its potential in cancer therapy research. Bmi1 has also been implicated in neurodegenerative disorders with connections to proteins like p21CIP1 affecting neuronal survival and aging.

style

left-column

Product protocols

style

left-column

Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 15386022, PubMed : 16359901, PubMed : 16714294, PubMed : 21772249, PubMed : 25355358, PubMed : 26151332, PubMed : 27827373). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed : 21772249, PubMed : 25355358). In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed : 15386022, PubMed : 21772249, PubMed : 26151332).

See full target information BMI1

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human BMI1 ELISA Kit

Sandwich ELISA - Human BMI1 ELISA Kit (AB272210)

Sandwich ELISA - Human BMI1 ELISA Kit (AB272210)

Sandwich ELISA - Human BMI1 ELISA Kit (AB272210)

Sandwich ELISA - Human BMI1 ELISA Kit (AB272210)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Product promise