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AB119583

Human BMP5 ELISA Kit

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(1 Publication)

Human BMP5 ELISA Kit is a Sandwich ELISA for the measurement of Human BMP5 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Bone morphogenetic protein 5, BMP-5, BMP5

1 Images
Sandwich ELISA - Human BMP5 ELISA Kit (AB119583)
  • sELISA

Supplier Data

Sandwich ELISA - Human BMP5 ELISA Kit (AB119583)

Representative Standard Curve using ab119583.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human BMP5 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BMP5 in cell culture supernatants, serum and bone tissue.

A BMP5 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BMP5 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BMP5 amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1683 pg/mL", "standardDeviation": "82.46", "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "242 pg/mL", "standardDeviation": "16.45", "coefficientOfVariability": "= 6.8" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1573 pg/mL", "standardDeviation": "69.21", "coefficientOfVariability": "= 4.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4290 pg/mL", "standardDeviation": "347.49", "coefficientOfVariability": "= 8.1" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4363 pg/mL", "standardDeviation": "314.13", "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "266 pg/mL", "standardDeviation": "20.74", "coefficientOfVariability": "= 7.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119583-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119583-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119583-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119583-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119583-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119583-CMP02", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plate Seal", "number":"AB119583-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119583-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human BMP5 antibody", "number":"AB119583-CMP08", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant Human BMP5 standard", "number":"AB119583-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human BMP5 antibody Microplate (12 x 8 wells)", "number":"AB119583-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119583-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cartilage and bone formation or neurogenesis (PubMed : 11580864, PubMed : 29321139). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed : 11580864). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes (PubMed : 11580864, PubMed : 29321139). Can also signal through non-canonical pathway such as MAPK p38 signaling cascade to promote chondrogenic differentiation (PubMed : 20402566). Promotes the expression of HAMP, this is repressed by its interaction with ERFE (PubMed : 30097509).
See full target information Bone morphogenetic protein 5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 17:2367-2379 PubMed34239363

2021

Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shiwei Xie,Chenyang Zhao,Wei Chen,Gengwu Li,Zhiwei Xiong,Xiangjun Tang,Fan Zhang,Heng Xiao
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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