Human BMP7 ELISA Kit
- Recombinant
- SimpleStep
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Human BMP7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BMP7 with a sensitivity of 0.2 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
OP1, BMP7, Bone morphogenetic protein 7, BMP-7, Osteogenic protein 1, OP-1
- sELISA
Supplier Data
Sandwich ELISA - Human BMP7 ELISA Kit (AB282294)
Example of human BMP-7 standard curve in Sample Diluent NS.
The BMP-7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human BMP7 ELISA Kit (AB282294)
Interpolated concentrations of native BMP-7 in human Daudi cell extract.
The concentrations of BMP-7 were measured in duplicate and interpolated from the BMP-7 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Reactivity data
Product details
Human BMP7 ELISA kit (ab282294) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human BMP7 protein in human serum, plasma, cell culture supernatant, and cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BMP7 with 0.20 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Bone Morphogenetic Protein 7 (BMP-7), also referred to as Osteogenic protein 1 and Eptotermin alfa, is a protein encoded in humans by the BMP7 gene. This protein is a member of the TGF beta superfamily, which plays a role in the recruitment and activation of SMAD family transcription factors. BMP7 is expressed as a preprotein which is proteolytically processed to form a subunit of a disulfide-linked homodimer. The active protein plays a role in bone, kidney, and brown adipose tissue development.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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