AB272470

Human BMP9 ELISA Kit

Recombinant
SimpleStep
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Human BMP9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BMP9 with a sensitivity of 0.97 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BMP9, GDF2, Growth/differentiation factor 2, GDF-2, Bone morphogenetic protein 9, BMP-9

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ELISA

Supplier Data

ELISA - Human BMP9 ELISA Kit (AB272470)

sELISA

Supplier Data

Sandwich ELISA - Human BMP9 ELISA Kit (AB272470)

Interpolated concentrations of native BMP9 in human serum, plasma and cell culture supernatant samples.

The concentrations of BMP9 were measured in duplicates, interpolated from the BMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 50%, and HepG2 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMP9 concentration was determined to be 213.4 pg/mL in serum, 166.3 pg/mL in plasma (citrate) and 37.3 pg/mL in HepG2 cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human BMP9 ELISA Kit (AB272470)

Example of human BMP9 standard curve in Sample Diluent NS.

The BMP9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.97 pg/mL

Range

4.69 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human BMP9 ELISA Kit ab272470 is a rapid single-wash 90-min Sandwich ELISA to measure Human BMP9 in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 0.97 pg/mL.

How the assay works

Human BMP9 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human BMP9 ELISA Kit ab272470 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-BMP9 antibody [EPR22898-235] - BSA and Azide free (Capture) ab272752
- Anti-BMP9 antibody [EPR22898-266] - BSA and Azide free (Detector) ab272752

Bone morphogenetic protein 9 (BMP9), also known as growth differentiation factor 2, is a protein encoded by the GDF2 gene and expressed in the liver. BMP9 is a member of the TGF-? superfamily that plays a role in angiogenesis as a potent inhibitor as well as a potent regulator for osteogenesis, glucose metabolism, and vascular tone. Studies have shown that mutations in BMP9 cause vascular disorders. Mouse, rat, cow, monkey share 96.4%, 94.5%, 92.7%, and 97.3% sequence homology, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "102 - 109 %", "average": "= 105" }, { "sample": "Serum", "range": "105 - 118 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "97 - 105 %", "average": "= 102" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.

See full target information GDF2

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human BMP9 ELISA Kit

ELISA - Human BMP9 ELISA Kit (AB272470)

Sandwich ELISA - Human BMP9 ELISA Kit (AB272470)

Sandwich ELISA - Human BMP9 ELISA Kit (AB272470)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Assay Kits

Product promise