AB193694
Human BNP ELISA Kit is a sandwich ELISA designed to quantify Human BNP with a sensitivity of 14 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 14 - 1000 pg/mL
- Cited in over 10 publications
View Alternative Names
Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB
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Supplier Data
Sandwich ELISA - Human BNP ELISA Kit (AB193694)
Standard curve using ab193694 BNP Human ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Complementary Products
Reactivity data
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Product details
Human BNP ELISA Kit ab193694 is a sandwich ELISA to measure Human BNP in serum, plasma, cell culture supernatant with a sensitivity of 14 pg/ml.
How the assay works
This assay employs an antibody specific for Human BNP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures BNP present in the samples. The wells are washed and biotinylated anti-Human BNP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of BNP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human BNP ELISA Kit ab193694 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.
How the assay works
This assay employs an antibody specific for Human BNP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures BNP present in the samples. The wells are washed and biotinylated anti-Human BNP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of BNP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human BNP ELISA Kit ab193694 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.
Precision
[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
Recovery
[ { "sample": "Serum", "range": "68 - 83 %", "average": "= 78.12" }, { "sample": "Plasma", "range": "67 - 107 %", "average": "= 80.96" }, { "sample": "Cell culture media", "range": "83 - 105 %", "average": "= 94.31" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed : 1672777, PubMed : 17372040, PubMed : 1914098, PubMed : 9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed : 1672777, PubMed : 17349887, PubMed : 17372040, PubMed : 21098034, PubMed : 25339504, PubMed : 9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed : 1914098, PubMed : 9458824). Binds the clearance receptor NPR3 (PubMed : 16870210).. NT-proBNP. May affect cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed : 17372040).. BNP(3-32). May have a role in cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP (PubMed : 17372040).
Publications (12)
Recent publications for all applications. Explore the full list and refine your search
Scientific reports 15:7704 PubMed40044985
2025
Alteration in Golgi apparatus fragmentation related genes in human dilated cardiomyopathy.
Applications
Unspecified application
Species
Unspecified reactive species
Irene González-Torrent,Isaac Giménez-Escamilla,Lorena Pérez-Carrillo,Marta Delgado-Arija,Manuel Portolés,Estefanía Tarazón,Esther Roselló-Lletí
Cancer medicine 12:13586-13598 PubMed37165917
2023
Aberrant expression of NPPB through YAP1 and TAZ activation in mesothelioma with Hippo pathway gene alterations.
Applications
Unspecified application
Species
Unspecified reactive species
Tatsuhiro Sato,Ken Akao,Ayuko Sato,Tohru Tsujimura,Satomi Mukai,Yoshitaka Sekido
BMC endocrine disorders 22:321 PubMed36529735
2022
Worsening effect of testosterone deficiency on males with heart failure with preserved ejection fraction.
Applications
Unspecified application
Species
Unspecified reactive species
Ahmed Hamam,Mahmoud Abou-Omar,Hanem Rabah,Haidy Khattab,Ahmed Alaarag
Frontiers in pharmacology 13:925276 PubMed35873593
2022
Dapagliflozin Protects Methamphetamine-Induced Cardiomyopathy by Alleviating Mitochondrial Damage and Reducing Cardiac Function Decline in a Mouse Model.
Applications
Unspecified application
Species
Unspecified reactive species
Shanqing He,Yajun Yao,Nan Yang,Youcheng Wang,Dishiwen Liu,Zhen Cao,Huiyu Chen,Yuntao Fu,Mei Yang,Songjun Wang,Guangjie He,Qingyan Zhao
Frontiers in cardiovascular medicine 8:691513 PubMed34395559
2021
The Discriminatory Ability of Renalase and Biomarkers of Cardiac Remodeling for the Prediction of Ischemia in Chronic Heart Failure Patients With the Regard to the Ejection Fraction.
Applications
Unspecified application
Species
Unspecified reactive species
Dijana Stojanovic,Valentina Mitic,Miodrag Stojanovic,Dejan Petrovic,Aleksandra Ignjatovic,Maja Milojkovic,Olivera Dunjic,Jelena Milenkovic,Vladmila Bojanic,Marina Deljanin Ilic
Cell 184:2167-2182.e22 PubMed33811809
2021
BET inhibition blocks inflammation-induced cardiac dysfunction and SARS-CoV-2 infection.
Applications
Unspecified application
Species
Unspecified reactive species
Richard J Mills,Sean J Humphrey,Patrick R J Fortuna,Mary Lor,Simon R Foster,Gregory A Quaife-Ryan,Rebecca L Johnston,Troy Dumenil,Cameron Bishop,Rajeev Rudraraju,Daniel J Rawle,Thuy Le,Wei Zhao,Leo Lee,Charley Mackenzie-Kludas,Neda R Mehdiabadi,Christopher Halliday,Dean Gilham,Li Fu,Stephen J Nicholls,Jan Johansson,Michael Sweeney,Norman C W Wong,Ewelina Kulikowski,Kamil A Sokolowski,Brian W C Tse,Lynn Devilée,Holly K Voges,Liam T Reynolds,Sophie Krumeich,Ellen Mathieson,Dad Abu-Bonsrah,Kathy Karavendzas,Brendan Griffen,Drew Titmarsh,David A Elliott,James McMahon,Andreas Suhrbier,Kanta Subbarao,Enzo R Porrello,Mark J Smyth,Christian R Engwerda,Kelli P A MacDonald,Tobias Bald,David E James,James E Hudson
Current medical research and opinion 36:909-919 PubMed32297799
2020
The partnership between renalase and ejection fraction as a risk factor for increased cardiac remodeling biomarkers in chronic heart failure patients.
Applications
Unspecified application
Species
Unspecified reactive species
Dijana Stojanovic,Valentina Mitic,Miodrag Stojanovic,Dejan Petrovic,Aleksandra Ignjatovic,Nikola Stefanovic,Tatjana Cvetkovic,Gordana Kocic,Vladmila Bojanic,Marina Deljanin Ilic
The Tohoku journal of experimental medicine 250:233-242 PubMed32295985
2020
Cardiac Remodeling Biomarkers as Potential Circulating Markers of Left Ventricular Hypertrophy in Heart Failure with Preserved Ejection Fraction.
Applications
Unspecified application
Species
Unspecified reactive species
Valentina T Mitic,Dijana R Stojanovic,Marina Z Deljanin Ilic,Miodrag M Stojanovic,Dejan B Petrovic,Aleksandra M Ignjatovic,Nikola Z Stefanovic,Gordana M Kocic,Vladmila V Bojanic
Biomaterials 233:119741 PubMed31927251
2020
Human cardiac fibrosis-on-a-chip model recapitulates disease hallmarks and can serve as a platform for drug testing.
Applications
Unspecified application
Species
Unspecified reactive species
Olya Mastikhina,Byeong-Ui Moon,Kenneth Williams,Rupal Hatkar,Dakota Gustafson,Omar Mourad,Xuetao Sun,Margaret Koo,Alan Y L Lam,Yu Sun,Jason E Fish,Edmond W K Young,Sara S Nunes
Disease markers 2019:7265160 PubMed31737132
2019
Association of Plasma Renalase and Left Ventricle Mass Index in Heart Failure Patients Stratified to the Category of the Ejection Fraction: A Pilot Study.
Applications
Unspecified application
Species
Unspecified reactive species
Dijana Stojanovic,Valentina Mitic,Dejan Petrovic,Miodrag Stojanovic,Aleksandra Ignjatovic,Nikola Stefanovic,Tatjana Cvetkovic,Vladmila Bojanic,Gordana Kocic,Marina Deljanin Ilic
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