Human BNP ELISA Kit ab193694 is used to quantify Human BNP with a sensitivity of 504 pg/ml.
Sandwich ELISA Kit
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- Cancer medicine 12:13586-135982023Aberrant expression of NPPB through YAP1 and TAZ activation in mesothelioma with Hippo pathway gene alterations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatsuhiro Sato,Ken Akao,Ayuko Sato,Tohru Tsujimura,Satomi Mukai,Yoshitaka SekidoPubMed 37165917
- BMC endocrine disorders 22:3212022Worsening effect of testosterone deficiency on males with heart failure with preserved ejection fraction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed Hamam,Mahmoud Abou-Omar,Hanem Rabah,Haidy Khattab,Ahmed AlaaragPubMed 36529735
- Frontiers in pharmacology 13:9252762022Dapagliflozin Protects Methamphetamine-Induced Cardiomyopathy by Alleviating Mitochondrial Damage and Reducing Cardiac Function Decline in a Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanqing He,Yajun Yao,Nan Yang,Youcheng Wang,Dishiwen Liu,Zhen Cao,Huiyu Chen,Yuntao Fu,Mei Yang,Songjun Wang,Guangjie He,Qingyan ZhaoPubMed 35873593
- Frontiers in cardiovascular medicine 8:6915132021The Discriminatory Ability of Renalase and Biomarkers of Cardiac Remodeling for the Prediction of Ischemia in Chronic Heart Failure Patients With the Regard to the Ejection Fraction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDijana Stojanovic,Valentina Mitic,Miodrag Stojanovic,Dejan Petrovic,Aleksandra Ignjatovic,Maja Milojkovic,Olivera Dunjic,Jelena Milenkovic,Vladmila Bojanic,Marina Deljanin IlicPubMed 34395559
- Cell 184:2167-2182.e222021BET inhibition blocks inflammation-induced cardiac dysfunction and SARS-CoV-2 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard J Mills,Sean J Humphrey,Patrick R J Fortuna,Mary Lor,Simon R Foster,Gregory A Quaife-Ryan,Rebecca L Johnston,Troy Dumenil,Cameron Bishop,Rajeev Rudraraju,Daniel J Rawle,Thuy Le,Wei Zhao,Leo Lee,Charley Mackenzie-Kludas,Neda R Mehdiabadi,Christopher Halliday,Dean Gilham,Li Fu,Stephen J Nicholls,Jan Johansson,Michael Sweeney,Norman C W Wong,Ewelina Kulikowski,Kamil A Sokolowski,Brian W C Tse,Lynn Devilée,Holly K Voges,Liam T Reynolds,Sophie Krumeich,Ellen Mathieson,Dad Abu-Bonsrah,Kathy Karavendzas,Brendan Griffen,Drew Titmarsh,David A Elliott,James McMahon,Andreas Suhrbier,Kanta Subbarao,Enzo R Porrello,Mark J Smyth,Christian R Engwerda,Kelli P A MacDonald,Tobias Bald,David E James,James E HudsonPubMed 33811809
- Current medical research and opinion 36:909-9192020The partnership between renalase and ejection fraction as a risk factor for increased cardiac remodeling biomarkers in chronic heart failure patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDijana Stojanovic et. al.PubMed 32297799
- The Tohoku journal of experimental medicine 250:233-2422020Cardiac Remodeling Biomarkers as Potential Circulating Markers of Left Ventricular Hypertrophy in Heart Failure with Preserved Ejection Fraction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValentina T Mitic,Dijana R Stojanovic,Marina Z Deljanin Ilic,Miodrag M Stojanovic,Dejan B Petrovic,Aleksandra M Ignjatovic,Nikola Z Stefanovic,Gordana M Kocic,Vladmila V BojanicPubMed 32295985
- Biomaterials 233:1197412020Human cardiac fibrosis-on-a-chip model recapitulates disease hallmarks and can serve as a platform for drug testing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlya Mastikhina,Byeong-Ui Moon,Kenneth Williams,Rupal Hatkar,Dakota Gustafson,Omar Mourad,Xuetao Sun,Margaret Koo,Alan Y L Lam,Yu Sun,Jason E Fish,Edmond W K Young,Sara S NunesPubMed 31927251
- Disease markers 2019:72651602019Association of Plasma Renalase and Left Ventricle Mass Index in Heart Failure Patients Stratified to the Category of the Ejection Fraction: A Pilot Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDijana Stojanovic,Valentina Mitic,Dejan Petrovic,Miodrag Stojanovic,Aleksandra Ignjatovic,Nikola Stefanovic,Tatjana Cvetkovic,Vladmila Bojanic,Gordana Kocic,Marina Deljanin IlicPubMed 31737132
- Critical care (London, England) 23:2592019Intravenous fluid resuscitation is associated with septic endothelial glycocalyx degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph A Hippensteel et. al.PubMed 31337421
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 14 - 1000 pg/mL
- Sensitivity
- = 14 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed:1672777, PubMed:17372040, PubMed:1914098, PubMed:9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed:1672777, PubMed:17349887, PubMed:17372040, PubMed:21098034, PubMed:25339504, PubMed:9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed:1914098, PubMed:9458824). Binds the clearance receptor NPR3 (PubMed:16870210). NT-proBNP. May affect cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed:17372040). BNP(3-32). May have a role in cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP (PubMed:17372040).
Alternative names
Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB
What's included?
Recommended products
Human BNP ELISA Kit ab193694 is used to quantify Human BNP with a sensitivity of 504 pg/ml.
Sandwich ELISA Kit
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 14 - 1000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 14 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 78.12 | Range 68 - 83 % |
Sample type Plasma | Average % = 80.96 | Range 67 - 107 % |
Sample type Cell culture media | Average % = 94.31 | Range 83 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's BNP Human ELISA Kit (ab193694) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BNP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human BNP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures BNP present in the samples. The wells are washed and biotinylated anti-Human BNP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of BNP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Sandwich ELISA - Human BNP ELISA Kit (ab193694)
Standard curve using ab193694 BNP Human ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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