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AB108708

Human Anti-Bordetella pertussis IgA ELISA Kit

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Human Anti-Bordetella pertussis IgA ELISA Kit is an indirect ELISA for the qualitative detection of IgA class antibodies against Bordetella pertussis in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Bordetella pertussis IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Bordetella pertussis in Human serum and plasma.

A 96-well plate has been precoated with Bordetella pertussis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Bordetella pertussis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bordetella pertussis IgA sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.18" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.94" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108708-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Bordetella pertussis anti-IgA HRP Conjugate", "number":"AB108708-CMP01", "productcode":"" }, { "size":"1 x 2 mL", "name":"Bordetella pertussis IgA Negative Control", "number":"AB108708-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"Bordetella pertussis IgA Positive Control", "number":"AB108708-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"Bordetella pertussis IgA Cut-off Control", "number":"AB108708-CMP04", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgA Sample Diluent", "number":"AB108708-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108708-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108708-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108708-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108708-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Bordetella pertussis (IgA) Coated Microplate (12 x 8 wells)", "number":"AB108708-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108708-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bordetella pertussis IgA also referred to as pertussis IgA is an antibody that plays a critical role in the immune response against Bordetella pertussis the bacterium responsible for whooping cough. This antibody is part of the immunoglobulin family and is known for its ability to bind antigens specifically related to pertussis bacteria. Although the exact molecular mass of pertussis IgA can exhibit slight variations due to its glycosylated nature it typically falls within the range common to IgA antibodies approximately 160 kDa. Pertussis IgA is expressed in mucosal surfaces particularly within the respiratory tract where the pathogen often establishes its infection.
Biological function summary

Antibodies like Bordetella pertussis IgA engage in the neutralization of toxins and prevention of bacterial adherence to epithelial cells. These antibodies although not part of a complex are essential in mucosal immunity providing a frontline defense against pathogens. The secretion of pertussis IgA at mucosal sites creates a barrier that impedes the colonization and proliferation of the Bordetella pertussis bacterium assisting in the containment and eventual clearance from the host.

Pathways

Antibodies like Bordetella pertussis IgA are integral to the adaptive immune response. They are involved in the humoral immune pathway effectively marking pathogens for destruction by other immune cells such as phagocytes and complement proteins. Bordetella pertussis IgA in its role within these pathways collaborates with other immunoglobulins like IgG and IgM each targeting various aspects of the pathogen lifecycle and contributing to a robust immune response.

Bordetella pertussis IgA is fundamentally implicated in combatting whooping cough (pertussis) infections. In individuals with compromised IgA production or function susceptibility to respiratory infections including chronic bronchitis may increase. During pertussis infection pertussis IgA interacts with other immune components like pertussis IgG facilitating a coordinated response aimed at neutralizing toxins clearing the bacteria and providing long-term immunity against future infections.
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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