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AB108709

Human Anti-Bordetella pertussis IgG ELISA Kit

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Human Anti-Bordetella pertussis IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Bordetella pertussis in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Bordetella pertussis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Bordetella pertussis in Human serum and plasma.

A 96-well plate has been precoated with Bordetella pertussis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Bordetella pertussis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bordetella pertussis IgG sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108709-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"Bordetella pertussis IgG Cut-off Control", "number":"AB108709-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"Bordetella pertussis IgG Negative Control", "number":"AB108709-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Bordetella pertussis anti-IgG HRP Conjugate", "number":"AB108709-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108709-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Bordetella pertussis Coated Microplate (12 x 8 wells)", "number":"AB108709-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108709-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108709-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108709-CMP09", "productcode":"" }, { "size":"1 x 2 mL", "name":"Bordetella pertussis IgG Positive Control", "number":"AB108709-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108709-CMP11", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108709-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bordetella pertussis IgG commonly referred to as pertussis IgG is an immunoglobulin that targets the Bordetella pertussis bacterium the causative agent of whooping cough. This IgG specifically binds to the Bordetella pertussis toxin neutralizing its effects and helping prevent colonization and infection in the respiratory tract. Immunoglobulin G (IgG) is a major antibody type in the body's immune response and is found expressed in blood interstitial fluid and tissues throughout the body. Bordetella pertussis toxin a protein with a molecular mass of approximately 105 kDa plays a significant role in the pathogen's virulence.
Biological function summary

The function of Bordetella pertussis IgG involves the human adaptive immune response where it aids in recognizing and neutralizing foreign pathogens like pertussis toxin. This IgG antibody does not form part of a larger complex itself but plays a critical role in the collective immune strategy. By binding with high affinity to the pertussis toxin the antibody blocks its interaction with host cells a step integral for the toxin’s ability to interfere with cellular processes and harm the host.

Pathways

Several components including Bordetella pertussis IgG play a role in the immune recognition and response pathways specifically the humoral immune response. This antibody works in conjunction with other immune proteins like memory B cells and helper T cells which aid in generating a stronger response upon future exposure to the pathogen. The action of Bordetella pertussis IgG is essential in the adaptive immune pathway reinforcing the overall defense mechanism against such bacterial pathogens.

Bordetella pertussis IgG is most closely associated with whooping cough a highly contagious respiratory disease. The presence and levels of this IgG indicate immunity level against the disease whether from vaccination or past infection. Furthermore insufficient levels of this antibody can leave individuals susceptible to Bordetella pertussis infections. Pertussis toxin and its antibody Bordetella pertussis IgG serve as key components in diagnostics and understanding the disease's clinical progression and immune evasion strategies.
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Product protocols

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Target data

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websiteProtocolBooklet
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