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AB108710

Human Anti-Bordetella pertussis IgM ELISA Kit

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(1 Publication)

Human Anti-Bordetella pertussis IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Bordetella pertussis in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Bordetella pertussis IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Bordetella pertussis in Human serum and plasma.

A 96-well plate has been precoated with Bordetella pertussis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Bordetella pertussis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bordetella pertussis IgM sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bordetella pertussis IgM also known as IgM Bordetella pertussis or anti-Bordetella pertussis IgM is an immunoglobulin class that plays a role in the early immune response against the Bordetella pertussis bacterium which causes whooping cough. IgM antibodies are an integral part of the body's first line of defense and have a pentameric structure with a high molecular weight of approximately 900 kDa. The expression of Bordetella pertussis IgM can be detected in the blood serum of infected individuals during the acute phase of the infection. This response indicates an active or recent exposure to the Bordetella pertussis pathogen.
Biological function summary

Bordetella pertussis IgM binds to antigens on the surface of Bordetella pertussis facilitating the elimination of the bacteria by the immune system. While not part of a larger protein complex they activate the complement system enhancing phagocytosis and lysing the bacteria. Their role is critical in initial infection control providing insights into infection dynamics and immune status of the host against this bacterium.

Pathways

Bordetella pertussis IgM influences the complement activation classical pathway an important component of the immune defense and is involved in the antibody-mediated immune response pathway. These pathways involve interactions with various immune proteins including complement proteins like C1q which facilitate the removal of the pathogen. The fast response of IgM antibodies aids in the containment and resolution of the infection prior to the activation of IgG antibodies which are more prevalent in long-term immunity.

Bordetella pertussis IgM is most strongly associated with whooping cough a highly contagious respiratory disease. The presence of these antibodies serves as a marker for diagnosing active Bordetella pertussis infection or recent vaccination. Though indirect this IgM does not connect primarily to other diseases its levels may guide clinicians in conjunction with other diagnostic proteins like pertussis toxin antigens to ensure appropriate treatment and control measures are in place.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ALTEX 37:47-63 PubMed31445503

2019

3D human microvessel-on-a-chip model for studying monocyte-to-endothelium adhesion under flow - application in systems toxicology.

Applications

Unspecified application

Species

Unspecified reactive species

Carine Poussin,Bart Kramer,Henriette L Lanz,Angelique Van den Heuvel,Alexandra Laurent,Thomas Olivier,Marjolein Vermeer,Dariusz Peric,Karine Baumer,Rémi Dulize,Emmanuel Guedj,Nikolai V Ivanov,Manuel C Peitsch,Julia Hoeng,Jos Joore
View all publications
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websiteProtocolBooklet
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