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AB108711

Human Anti-Borrelia burgdorferi IgM ELISA Kit

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(1 Publication)

Human Anti-Borrelia burgdorferi IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Borrelia burgdorferi in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Borrelia burgdorferi IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF (cerebrospinal fluid).

A 96-well plate has been precoated with recombinant Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgM sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 14, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108711-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"Borrelia burgdorferi IgM Cut-off Control", "number":"AB108711-CMP04", "productcode":"" }, { "size":"1 x 2 mL", "name":"Borrelia burgdorferi IgM Positive Control", "number":"AB108711-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Borrelia burgdorferi anti-IgM HRP Conjugate", "number":"AB108711-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Borrelia burgdorferi (IgM) Coated Microplate (12 x 8 wells)", "number":"AB108711-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108711-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108711-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108711-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108711-CMP09", "productcode":"" }, { "size":"1 x 2 mL", "name":"Borrelia burgdorferi IgM Negative Control", "number":"AB108711-CMP05", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgM Sample Diluent", "number":"AB108711-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108711-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Borrelia burgdorferi IgM sometimes referred to as B. burgdorferi IgM is an immunoglobulin M (IgM) antibody that specifically targets the outer surface proteins of the Borrelia burgdorferi bacterium. This bacterium is the primary causative agent of Lyme disease. Mechanically these antibodies are detectable using techniques like the Borrelia test kit or Lyme ELISA which identify the presence of Borrelia burgdorferi IgM in the blood. The detection of IgM antibodies indicates an early immune response and suggests that the body recently encountered the pathogen. These antibodies are typically expressed in the serum of infected individuals.
Biological function summary

The identification of Borrelia burgdorferi IgM involves the host’s immune response against the bacterial pathogen responsible for Lyme disease. These IgM antibodies play an important role in the initial immune response by binding to antigens present on the Borrelia bacterium. This binding initiates immune reactions like complement activation which aids in the elimination of the pathogen. Borrelia-specific IgM antibodies are not part of a larger protein complex but rather indicate the early phase of humoral immunity.

Pathways

Borrelia burgdorferi IgM involvement connects to key immune pathways such as the complement pathway and acute phase response. Detection of these antibodies signals the activation of the host's immune system indicating an ongoing or recent immune defense against the infection. While not directly interacting with specific proteins the presence of Borrelia burgdorferi IgM suggests interactions with components of these immune pathways which include other immune effectors and molecules.

Borrelia burgdorferi IgM is significantly associated with Lyme disease an infectious condition transmitted through the bite of infected Ixodes ticks. The detection of these IgM antibodies can help in diagnosing Lyme disease during its early stages. Furthermore the persistence of IgM alongside IgG antibodies can indicate chronic infection or re-infection playing a role in post-treatment Lyme disease symptoms. The interaction of Borrelia burgdorferi IgM with the immune system can also lead to complications in diagnosing other spirochetal infections due to potential cross-reactivity with antigens from similar pathogens.
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Product protocols

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Target data

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Publications (1)

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Acta tropica 140:188-92 PubMed25239124

2014

Lyme borreliosis: A neglected zoonosis in Egypt.

Applications

Unspecified application

Species

Unspecified reactive species

Rehab A Elhelw,Mona I El-Enbaawy,Ahmed Samir
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websiteProtocolBooklet
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