AB315039

Human Brevican ELISA Kit

SimpleStep
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Human Brevican ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Brevican with a sensitivity of 2.634 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BEHAB, CSPG7, UNQ2525/PRO6018, BCAN, Brevican core protein, Brain-enriched hyaluronan-binding protein, Chondroitin sulfate proteoglycan 7

7 Images

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

sELISA

Supplier Data

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Example of Human Brevican standard curve in 1X Wash Buffer PT. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Interpolated concentration of native Brevican was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 100% SH-SY5Y cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Interpolated concentration of native Brevican was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Human serum 25%, plasma (EDTA) 50%, and 25% plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NSE.

sELISA

Supplier Data

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Example of Human Brevican standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Serum of ten individual healthy Human male and healthy Human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Brevican concentration was determined to be 450.8 pg/mL with a range of 273.5 – 658.0 pg/mL

sELISA

Supplier Data

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Example of Human Brevican standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Interpolated concentration of native Brevican was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 2.25% CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, EDTA Plasma, Cerebral Spinal Fluid, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.634 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Brevican SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Brevican protein in Serum, Hep Plasma, EDTA Plasma, Cerebral Spinal Fluid, Cell culture supernatant . Quantitate Human Brevican with 2.634 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.76" } ]

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Recovery

[ { "sample": "Serum", "range": "86 - 88 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "78 - 89 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "90 - 95 %", "average": "= 92" }, { "sample": "Cerebral Spinal Fluid", "range": "94 - 107 %", "average": "= 100" }, { "sample": "Cell culture supernatant", "range": "87 - 119 %", "average": "= 106" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315039-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315039-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB315039-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315039-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315039-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315039-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Brevican Lyophilized Recombinant Protein", "number":"AB315039-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB315039-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Brevican Capture Antibody", "number":"AB315039-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Brevican Detector Antibody", "number":"AB315039-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315039-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315039-CMP11", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Brevican also known as BEHAB is a prominent extracellular matrix proteoglycan. This molecule with a molecular weight of approximately 145 kDa mainly expresses in the central nervous system. It is found especially in the brain and contributes significantly to neural network organization. Brevican is a major component of perineuronal nets specialized structures that surround neurons and support their functions.

Biological function summary

Brevican regulates cell adhesion migration and axonal guidance. As part of a proteoglycan complex it interacts with other matrix components and cell surface receptors to influence cell signaling. Its modular structure allows it to bind with various molecules which can modulate extracellular matrix composition and neuronal connectivity. This regulation is vital for maintaining synaptic plasticity and brain tissue integrity.

Pathways

Brevican plays an important role in neural development and plasticity. It participates in the extracellular matrix pathway that modulates neuronal-circuit dynamics. This pathway involves interactions with molecules like versican and neurocan which are integral to synaptic stabilization and remodeling. Brevican's interactions within these pathways help coordinate extracellular and intracellular processes that underlie neural network adaptations.

Brevican has strong associations with brain tumors and neurodegenerative diseases. Research indicates its altered expression in gliomas where it may influence tumor growth and cell invasion. Changes in brevican levels also link to Alzheimer's disease where it might affect amyloid plaque formation. In these conditions brevican's interactions with proteins such as tenascin-R and chondroitin sulfate proteoglycans could impact disease progression and cellular responses.

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Product protocols

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Target data

May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.

See full target information BCAN

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Brevican ELISA Kit

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Sandwich ELISA - Human Brevican ELISA Kit (AB315039)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise