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AB108712

Human Anti-Brucella IgG ELISA Kit

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(2 Publications)

Human Anti-Brucella IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Brucella in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Brucella IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Brucella in Human serum and plasma.

A 96-well plate has been precoated with Brucella antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Brucella-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Brucella IgG sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 23, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108712-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Brucella IgG Negative Control", "number":"AB108712-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108712-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108712-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Brucella (antigen) Coated Microplate (12 x 8 wells)", "number":"AB108712-CMP03", "productcode":"" }, { "size":"1 x 2 mL", "name":"Brucella IgG Positive Control", "number":"AB108712-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108712-CMP09", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108712-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108712-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Brucella anti-IgG HRP Conjugate", "number":"AB108712-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108712-CMP11", "productcode":"" }, { "size":"1 x 3 mL", "name":"Brucella IgG Cut-off Control", "number":"AB108712-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Brucella IgG known also as Immunoglobulin G for Brucella detection possesses an important role in the immune response against Brucella species which are intracellular bacterial pathogens. Brucella IgG is an antibody class involved in identifying and neutralizing these bacteria. Its molecular mass typically falls within the range of 150 kDa characteristic of IgG antibodies. This target is usually expressed in the serum of infected individuals and serves as an indicator of chronic or past infection. Various diagnostic assays including enzyme-linked immunosorbent assays (ELISA) frequently employ this immunoglobulin to measure levels of specific antibodies present in the patient’s system.
Biological function summary

Brucella IgG contributes significantly to the immune defense by facilitating opsonization and enhancing phagocytosis of Brucella organisms by immune cells. This antibody may not form part of a larger complex but works in concert with other components of the immune system like macrophages and cytokines. The presence of Brucella IgG can indicate an adaptive immune response maturation but not necessarily active bacterial replication. Consequently monitoring IgG levels can provide insights into disease progression and the individual's immune status in response to Brucella exposure.

Pathways

Detailed knowledge of Brucella IgG's functional role should consider its involvement in the humoral immune response. It is pivotal within the adaptive immune system pathways particularly in processes recognizing and neutralizing pathogens. Brucella IgG synergizes with complement proteins enhancing bacteriolysis and clearance of Brucella organisms. Another related pathway includes its interaction with Fc receptors on effector cells triggering phagocytosis upon pathogen binding which is essential for pathogen elimination and infection control.

Comprehensive understanding emphasizes Brucella IgG's relevance in diagnosing and monitoring brucellosis an infection requiring careful management due to its persistence and possible complications. Heightened levels of Brucella IgG correlate with chronic brucellosis aiding in distinguishing it from acute infection phases. This antibody also connects to other proteins like IgM in serological profiling assisting clinicians in comprehensive disease evaluation and therapy response assessment. Understanding such disease-related dynamics ensures effective brucellosis management and potentially minimizes widespread transmission risks.
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scandinavian journal of immunology 92:e12877 PubMed32145090

2020

Adipose-derived mesenchymal stromal cells suppress osteoclastogenesis and bone erosion in collagen-induced arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Chang,Chao Li,Yunjie Lu,Rui Geng,Ji-Nan Wei,Jun-Zheng Hu

Proceedings of the National Academy of Sciences of 113:9822-7 PubMed27535933

2016

Lysine methylation represses p53 activity in teratocarcinoma cancer cells.

Applications

WB

Species

Human

Jiajun Zhu,Zhixun Dou,Morgan A Sammons,Arnold J Levine,Shelley L Berger
View all publications
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