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AB108713

Human Anti-Brucella IgM ELISA Kit

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Human Anti-Brucella IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Brucella in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Brucella IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Brucella in Human serum and plasma.

A 96-well plate has been precoated with Brucella antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Brucella-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Brucella IgM sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108713-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Brucella anti-IgM HRP Conjugate", "number":"AB108713-CMP01", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgM Sample Diluent", "number":"AB108713-CMP06", "productcode":"" }, { "size":"1 x 2 mL", "name":"Brucella IgM Negative Control", "number":"AB108713-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"Brucella IgM Cut-off Control", "number":"AB108713-CMP03", "productcode":"" }, { "size":"1 x 2 mL", "name":"Brucella IgM Positive Control", "number":"AB108713-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108713-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108713-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108713-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108713-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Brucella (IgM) Coated Microplate (12 x 8 wells)", "number":"AB108713-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108713-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Brucella IgM is an immunoglobulin associated with the early immune response to Brucella infection. Also known as Immunoglobulin M IgM this antibody is the first responder during the acute phase of infection. IgM antibodies are larger in size compared to other antibodies having a mass of approximately 900 kDa due to their pentameric structure. They are typically expressed on the surface of B cells and are found in the bloodstream. The presence of Brucella IgM indicates initial immune activation against Brucella bacteria which can cause brucellosis in humans.
Biological function summary

Brucella IgM serves as an essential component of the body's defense mechanism against Brucella species. It plays a major role in the immune response by recognizing and binding to the Brucella antigen effectively marking these pathogens for destruction by other immune cells. As part of the immune complex IgM functions are integral in neutralizing and agglutinating pathogens. By forming immune complexes IgM enhances phagocytosis by macrophages and neutrophils which are critical in clearing the infection from the host.

Pathways

Brucella IgM antibodies participate in the complement activation pathway which is important for opsonization and lysis of bacterial cells. The complement system involving proteins such as C1q C3 and C4 interacts directly with IgM leading to a cascade of reactions that amplify the immune response against Brucella. This pathway is tightly associated with humoral immune response where other antibodies like IgG although later in the disease process work in conjunction to provide longer-lasting immunity.

Brucella IgM is strongly connected to brucellosis a zoonotic disease that affects various organ systems in humans. The detection of IgM antibodies serves as an important diagnostic marker for acute brucellosis infection. Besides direct involvement with brucellosis the presence of Brucella IgM can influence autoimmune responses due to molecular mimicry potentially leading to conditions like reactive arthritis. In these scenarios Brucella IgM may interplay with host proteins such as HLA-B27 which is implicated in autoimmune-related inflammation complicating the clinical picture.
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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