AB231931

Human BST2 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Human BST2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BST2 with a sensitivity of 0.28 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD317, Bone marrow stromal antigen 2, BST-2, HM1.24 antigen, Tetherin, BST2

5 Images

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

sELISA

Supplier Data

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

Interpolated concentrations of native BST2 in human serum and plasma samples.

The concentrations of BST2 were measured in duplicates, interpolated from the BST2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (heparin) 100%, plasma (EDTA) 50%, and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BST2 concentration in the neat samples was determined to be 172 pg/mL in serum, 148 pg/mL in plasma (heparin), 133 pg/mL in plasma (EDTA), and 206 pg/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BST2 concentration was determined to be 221 pg/mL with a range of 74 – 672 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

Interpolated concentrations of spiked BST2 in RPMI media and of native BST2 in cell extract samples.

The concentrations of BST2 were measured in duplicate and interpolated from the BST2 standard curve and corrected for sample dilution. Undiluted samples are as follows : RPMI media 50%, A431 cell extract 10 μg/mL, and HeLa cell extract 30 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

Example of human BST2 standard curve in 1X Cell Extraction Buffer PTR.

The BST2 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

Example of human BST2 standard curve in Sample Diluent NS.

The BST2 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.28 pg/mL

Range

5 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human BST2 ELISA Kit (ab231931) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BST2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BST2 with NULL NULL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "99 - 103 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "99 - 104 %", "average": "= 102" }, { "sample": "Cell culture extracts", "range": "109 - 117 %", "average": "= 113" }, { "sample": "Heparin Plasma", "range": "93 - 1010 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "98 - 103 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "99 - 100 %", "average": "= 100" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab231931-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB231931-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB231931-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB231931-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BST2 Detector Antibody", "number":"AB231931-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB231931-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB231931-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB231931-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB231931-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB231931-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BST2 Capture Antibody", "number":"AB231931-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human BST2 Lyophilized Recombinant Protein", "number":"AB231931-CMP09", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted (PubMed : 18200009, PubMed : 18342597, PubMed : 19036818, PubMed : 19879838, PubMed : 20019814, PubMed : 20399176, PubMed : 20419159, PubMed : 20940320, PubMed : 21529378, PubMed : 22520941). Its target viruses belong to diverse families, including retroviridae : human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae : hepatitis C virus (HCV), filoviridae : ebola virus (EBOV) and marburg virus (MARV), arenaviridae : lassa virus (LASV) and machupo virus (MACV), herpesviridae : kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae : vesicular stomatitis virus (VSV), orthomyxoviridae : influenza A virus, paramyxoviridae : nipah virus, and coronaviridae : SARS-CoV (PubMed : 18200009, PubMed : 18342597, PubMed : 19179289, PubMed : 19879838, PubMed : 20399176, PubMed : 20419159, PubMed : 20686043, PubMed : 20943977, PubMed : 21529378, PubMed : 21621240, PubMed : 22520941, PubMed : 26378163, PubMed : 31199522). Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration (PubMed : 22065321). Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection (PubMed : 19564354, PubMed : 26172439). Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Isoform 1 and isoform 2 are both effective viral restriction factors but have differing antiviral and signaling activities (PubMed : 23028328, PubMed : 26172439). Isoform 2 is resistant to HIV-1 Vpu-mediated degradation and restricts HIV-1 viral budding in the presence of Vpu (PubMed : 23028328, PubMed : 26172439). Isoform 1 acts as an activator of NF-kappa-B and this activity is inhibited by isoform 2 (PubMed : 23028328).

See full target information BST2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer gene therapy 32:38-50 PubMed39558134

2024

Serum small extracellular vesicles-derived BST2 as a biomarker for papillary thyroid microcarcinoma promotes lymph node metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Cao,Yuanyang Wang,Jianqiang Wu,Xiaoyue Tang,Zhihong Qian,Zejian Zhang,Rui Liu,Peng Liu,Zepeng Li,Xiequn Xu,Ziwen Liu

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human BST2 ELISA Kit

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

Sandwich ELISA - Human BST2 ELISA Kit (AB231931)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Assay Kits

Product promise