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AB326409

Human BST2 ELISA Kit, Chemiluminescent

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Human BST2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human BST2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.46 - 1000 pg/ml with a sensitivity of 0.15pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

CD317, Bone marrow stromal antigen 2, BST-2, HM1.24 antigen, Tetherin, BST2

6 Images
Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)
  • sELISA

Lab

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)
  • sELISA

Lab

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)

Standard curve comparison between the Chemiluminescent Human BST2 SimpleStep ELISA and colorimetric Human BST2 SimpleStep ELISA (ab231931) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)
  • sELISA

Lab

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)

Dynamic range comparison between the Chemiluminescent Human BST2 SimpleStep ELISA, colorimetric Human BST2 SimpleStep ELISA (ab231931), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)
  • sELISA

Supplier Data

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean BST2 concentration was determined to be 221 pg/mL with a range of 74 – 672 pg/mL.

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)
  • sELISA

Supplier Data

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)

Interpolated concentrations of spiked BST2 in RPMI media and of native BST2 in cell extract samples.

The concentrations of BST2 were measured in duplicate and interpolated from the BST2 standard curve and corrected for sample dilution. Undiluted samples are as follows : RPMI media 50% A431 cell extract 10 µg/mL and HeLa cell extract 30 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2).

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)
  • sELISA

Supplier Data

Sandwich ELISA - Human BST2 ELISA Kit, Chemiluminescent (AB326409)

Interpolated concentrations of native BST2 in human serum and plasma samples.

The concentrations of BST2 were measured in duplicates interpolated from the BST2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100% plasma (heparin) 100% plasma (EDTA) 50% and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean BST2 concentration in the neat samples was determined to be 172 pg/mL in serum 148 pg/mL in plasma (heparin) 133 pg/mL in plasma (EDTA) and 206 pg/mL in plasma (citrate).

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.15 pg/mL

Range

0.46 - 1000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human BST2 ELISA Kit, Chemiluminescent (ab326409) is a rapid single-wash sandwich 55-min ELISA to measure Human BST2 in Serum, EDTA Plasma, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 0.15 pg/mL.

How the assay works

Human BST2 ELISA Kit, Chemiluminescent (ab326409) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human BST2 (ab326409) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-BST2 antibody [EPR20202-150] - BSA and Aside free (Capture) ab242967
- Anti-BST2 antibody [EPR20202-169] - BSA and Aside free (Detector) ab243001
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.2" } ]
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Recovery

[ { "sample": "Serum", "range": "99 - 103 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "99 - 104 %", "average": "= 102" }, { "sample": "Cell culture extracts", "range": "109 - 117 %", "average": "= 113" }, { "sample": "Heparin Plasma", "range": "93 - 1010 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "98 - 103 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "99 - 100 %", "average": "= 100" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted (PubMed : 18200009, PubMed : 18342597, PubMed : 19036818, PubMed : 19879838, PubMed : 20019814, PubMed : 20399176, PubMed : 20419159, PubMed : 20940320, PubMed : 21529378, PubMed : 22520941). Its target viruses belong to diverse families, including retroviridae : human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae : hepatitis C virus (HCV), filoviridae : ebola virus (EBOV) and marburg virus (MARV), arenaviridae : lassa virus (LASV) and machupo virus (MACV), herpesviridae : kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae : vesicular stomatitis virus (VSV), orthomyxoviridae : influenza A virus, paramyxoviridae : nipah virus, and coronaviridae : SARS-CoV (PubMed : 18200009, PubMed : 18342597, PubMed : 19179289, PubMed : 19879838, PubMed : 20399176, PubMed : 20419159, PubMed : 20686043, PubMed : 20943977, PubMed : 21529378, PubMed : 21621240, PubMed : 22520941, PubMed : 26378163, PubMed : 31199522). Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration (PubMed : 22065321). Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection (PubMed : 19564354, PubMed : 26172439). Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Isoform 1 and isoform 2 are both effective viral restriction factors but have differing antiviral and signaling activities (PubMed : 23028328, PubMed : 26172439). Isoform 2 is resistant to HIV-1 Vpu-mediated degradation and restricts HIV-1 viral budding in the presence of Vpu (PubMed : 23028328, PubMed : 26172439). Isoform 1 acts as an activator of NF-kappa-B and this activity is inhibited by isoform 2 (PubMed : 23028328).
See full target information BST2
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Product promise

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