AB323689

Human BTN3A2 + BTN3A3 ELISA Kit- Extracellular

Recombinant
SimpleStep
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Human BTN3A2 + BTN3A3 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human BTN3A2 + BTN3A3 with a sensitivity of 1.8 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

BT3.2, BTF3, BTF4, BTN3A2, Butyrophilin subfamily 3 member A2

5 Images

Sandwich ELISA - Human BTN3A2 + BTN3A3 ELISA Kit- Extracellular (AB323689)

sELISA

Supplier Data

Sandwich ELISA - Human BTN3A2 + BTN3A3 ELISA Kit- Extracellular (AB323689)

Interpolated concentration of native BTN3A2 + BTN3A3 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human BTN3A2 + BTN3A3 ELISA Kit- Extracellular (AB323689)

Example of human BTN3A2 + BTN3A3 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human BTN3A2 + BTN3A3 ELISA Kit- Extracellular (AB323689)

Interpolated concentration of native BTN3A2 + BTN3A3 was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human BTN3A2 + BTN3A3 ELISA Kit- Extracellular (AB323689)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean BTN3A2 + BTN3A3 concentration was determined to be 391 pg/mL with a range of 220 – 614 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human BTN3A2 + BTN3A3 ELISA Kit- Extracellular (AB323689)

Example of human BTN3A2 + BTN3A3 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.8 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human BTN3A2 + BTN3A3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BTN3A2 + BTN3A3 protein in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell culture media. Quantitate Human BTN3A2 + BTN3A3 with 1.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.2" } ]

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Recovery

[ { "sample": "Serum", "range": "94 - 98 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "81 - 85 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "98 - 111 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "86 - 90 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "105 - 110 %", "average": "= 107" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Plays a role in T-cell responses in the adaptive immune response. Inhibits the release of IFNG from activated T-cells.

See full target information BTN3A2

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websiteProtocolBooklet

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Product promise

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