Human BTN3A2 + BTN3A3 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human BTN3A2 + BTN3A3 with a sensitivity of 2.4 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 12.5 - 800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a role in T-cell responses in the adaptive immune response. Inhibits the release of IFNG from activated T-cells.
Alternative names
BT3.2, BTF3, BTF4, BTN3A2, Butyrophilin subfamily 3 member A2
What's included?
Human BTN3A2 + BTN3A3 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human BTN3A2 + BTN3A3 with a sensitivity of 2.4 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 12.5 - 800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. = 2.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. = 1.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 107 | Range 102 - 113 % |
Sample type Tissue Extracts | Average % = 101 | Range 94 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human BTN3A2 + BTN3A3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BTN3A2 + BTN3A3 protein in Cell Lysate, Tissue extracts. Quantitate Human BTN3A2 + BTN3A3 with 2.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human BTN3A2 + BTN3A3 ELISA Kit- Intracellular (ab323690)
Interpolated concentration of native BTN3A2 + BTN3A3 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100 µg/mL Jurkat cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human BTN3A2 + BTN3A3 ELISA Kit- Intracellular (ab323690)
Example of human BTN3A2 + BTN3A3 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human BTN3A2 + BTN3A3 ELISA Kit- Intracellular (ab323690)
Interpolated concentration of native BTN3A2 + BTN3A3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: Jurkat cell extract 100 µg/mL, HUVEC cell extract 500 µg/mL, and spleen tissue extract 25 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human BTN3A2 + BTN3A3 ELISA Kit- Intracellular (ab323690)
Example of human BTN3A2 + BTN3A3 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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