Human Butyrylcholinesterase ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Butyrylcholinesterase with a sensitivity of 350.255 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1171.875 - 75000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 350.255 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Alternative names
CHE1, BCHE, Cholinesterase, Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II, Pseudocholinesterase
What's included?
Human Butyrylcholinesterase ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Butyrylcholinesterase with a sensitivity of 350.255 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1171.875 - 75000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 350.255 pg/mL
- General recovery
- 110%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 4.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 5.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 110 | Range 108 - 113 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Butyrylcholinesterase SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Butyrylcholinesterase protein in Cell Lysate. Quantitate Human Butyrylcholinesterase with 350.26 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human Butyrylcholinesterase ELISA Kit- Intracellular (ab322430)
Example of human Butyrylcholinesterase standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Butyrylcholinesterase ELISA Kit- Intracellular (ab322430)
Example of human Butyrylcholinesterase standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Butyrylcholinesterase ELISA Kit- Intracellular (ab322430)
Interpolated concentration of native Butyrylcholinesterase was measured in duplicate at different sample concentrations. Undiluted samples are 50 ?g/mL LNCaP cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human Butyrylcholinesterase ELISA Kit- Intracellular (ab322430)
Interpolated concentration of native Butyrylcholinesterase was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50 ?g/mL LNCaP cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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