Human c-Myc ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human c-Myc in Human in Plasma, Cell culture supernatant, Serum samples.
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- Toxics 11:2023A Proposed Association between Improving Energy Metabolism of HepG2 Cells by Plant Extracts and Increasing Their Sensitivity to Doxorubicin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTamer A Al-Shafie,Engy A Mahrous,Mustafa Shukry,Mohammad Y Alshahrani,Samah F Ibrahim,Liana Fericean,Afaf Abdelkader,Mennatallah A AliPubMed 36851057
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.614 - 150 ng/mL
- Sensitivity
- = 0.62 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
Alternative names
BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39
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Human c-Myc ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human c-Myc in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.614 - 150 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.62 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 73.27 | Range 69 - 80 % |
Sample type Plasma | Average % = 72.19 | Range 69 - 80 % |
Sample type Cell culture media | Average % = 89.52 | Range 84 - 95 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human c-Myc ELISA Kit (ab277452) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human c-Myc in serum (human c-Myc concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human c-Myc coated on a 96-well plate. Standards and samples are pipetted into the wells and Human c-Myc present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human c-Myc antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human c-Myc bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
- Biological function summary
C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.
- Pathways
C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.
- Associated diseases and disorders
C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
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ELISA - Human c-Myc ELISA Kit (ab277452)
These standard curves are for demonstration only. A standard curve must be run with each assay.
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