AB323521

Human c-Myc ELISA Kit

SimpleStep
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Human c-Myc ELISA Kit is a single-wash 90-min Simplestep used to quantify Human c-Myc with a sensitivity of 36.684 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

5 Images

Sandwich ELISA - Human c-Myc ELISA Kit (AB323521)

sELISA

Supplier Data

Sandwich ELISA - Human c-Myc ELISA Kit (AB323521)

Human c-Myc isoforms reactivity.

Human recombinant c-MYC isoform 1 and isoform 2 protein reactivity. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human c-Myc ELISA Kit (AB323521)

Interpolated concentrations of human c-Myc in HeLa cell extract in 96-well vs. 384-well plates.

Interpolated concentration of native c-Myc was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are HeLa cell extract 125 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human c-Myc ELISA Kit (AB323521)

Interpolated concentrations of human c-Myc in HeLa cell extract, Jurkat cell extract, and lung tissue extract.

Interpolated concentration of native c-Myc was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HeLa cell extract 500 µg/mL, Jurkat cell extract 150 µg/mL and lung tissue extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human c-Myc ELISA Kit (AB323521)

Example of human c-Myc standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.

Example of human c-Myc standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human c-Myc ELISA Kit (AB323521)

Example of human c-Myc standard curve in 1X Cell Extraction Buffer PTR.

Example of human c-Myc standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 36.684 pg/mL

Range

140.625 - 9000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human c-Myc SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of c-Myc protein in Cell Lysate, Tissue extracts. Quantitate Human c-Myc with 36.684 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Single-wash protocol reduces assay time to 90 minutes or less
High sensitivity, specificity and reproducibility from superior antibodies
Fully validated in biological samples
96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "113 - 120 %", "average": "= 117" }, { "sample": "Tissue Extracts", "range": "95 - 101 %", "average": "= 98" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).

See full target information MYC

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websiteProtocolBooklet

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Product promise

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