Human C-Peptide ELISA Kit is a single-wash 90-min Simplestep used to quantify Human C-Peptide with a sensitivity of 1.45 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture media, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.13 - 200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.45 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Alternative names
Insulin, INS
What's included?
Recommended products
Human C-Peptide ELISA Kit is a single-wash 90-min Simplestep used to quantify Human C-Peptide with a sensitivity of 1.45 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture media, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.13 - 200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.45 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 2.6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 3.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 105 | Range 100 - 110 % |
Sample type Urine | Average % = 103 | Range 101 - 107 % |
Sample type Cell culture media | Average % = 90 | Range 88 - 92 % |
Sample type Heparin Plasma | Average % = 92 | Range 85 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human C-Peptide ELISA Kit ab260064 is a rapid single-wash 90-min sandwich ELISA kit to measure Human C-Peptide in Serum, Heparin Plasma, Urine, Cell culture media, and Heparin Plasma. This Simplestep sensitivity is 1.45 pg/mL.
How the assay works
Human C-Peptide SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
This immunoassay is calibrated against a highly purified human C-Peptide. The NIBSC/WHO unclassified purified human C-Peptide preparation 13/146 was evaluated in this kit. The dose response curve of the unclassified standard C-Peptide parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human C-Peptide kit to approximate NIBSC mg/mL, use the equation below.
NIBSC (13/146) approximate value (mg/mL) = 1.077 x SimpleStep Human C-Peptide value (mg/mL).
Human C-Peptide ELISA Kit ab260064 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-C Peptide antibody [EPR22500-5] - BSA and Azide free (Capture) Anti-C Peptide antibody [EPR22500-5] - BSA and Azide free (Capture) ab259549
- Anti-C Peptide antibody [EPR22498-2] - BSA and Azide free (Detector) Anti-C Peptide antibody [EPR22498-2] - BSA and Azide free (Detector) ab259550
Related and Recommended products
Human C-Peptide ELISA Kit ab260064 is commonly used to measure C-peptide as a marker of insulin metabolism in obesity and diabetes.
Other ELISA kits to measure metabolic markers include:
- Human Proinsulin ELISA Kit Human Proinsulin ELISA Kit ab242235
- Human Insulin ELISA Kit Human Insulin ELISA Kit ab278123
- Human GLP1 (7-36) ELISA Kit Human GLP1 (7-36) ELISA Kit ab184857
C-peptide of insulin is a cleavage product produced during processing of the insulin pro-hormone to the mature insulin molecule. Proinsulin is cleaved when it is released from the pancreas into the blood - one C-peptide for each insulin molecule. C-Peptide has been shown to bind to a variety of cell types, and in vivo animal diabetes models have shown positive effects on kidney and nerve function.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C-peptide also known as connecting peptide is a short polypeptide chain consisting of 31 amino acids with a molecular weight of about 3 kDa. It results from enzymatic cleavage during the conversion of proinsulin to insulin and is co-secreted equimolarly with insulin by beta cells of the pancreas. C-peptide does not undergo significant liver metabolism making its levels a meaningful indicator of endogenous insulin production. This peptide is important due to its ability to serve as a biomarker for beta cell function giving insights into the body's insulin production capacity. Commercially available assays for its measurement include C-peptide ELISA and C-peptide ELISA kit important in both clinical and research settings.
- Biological function summary
C-peptide plays a role beyond being a mere byproduct of insulin synthesis. Experiments have shown it binds to cell membranes indicating it functions independently and is more than part of the insulin processing complex. Studies suggest C-peptide may have physiological effects such as improving blood flow and possessing anti-inflammatory properties. Its ability to bind likely involves specific interactions that suggest possible receptors a subject of ongoing research. Detection methods like peptide ELISA and peptide test kit further facilitate studying its biological activity and potential therapeutic implications.
- Pathways
C-peptide is closely linked to the metabolic insulin signaling pathway. This pathway is integral in glucose homeostasis and involves proteins such as insulin receptor and glucose transporter. Although not directly involved in the receptor-mediated actions of insulin C-peptide's presence alongside insulin shortly after release places it within this critical pathway. Another related pathway includes peptide hormone responses where C-peptide might influence vasodilation or cellular signaling revealing potential avenues for regulating metabolic conditions.
- Associated diseases and disorders
C-peptide is most notably associated with diabetes mellitus specifically type 1 and type 2. Its measurement helps to distinguish between the types of diabetes as it reflects the pancreas's ability to produce insulin. Lower levels often correlate with type 1 diabetes due to autoimmune destruction of beta cells whereas type 2 diabetes may still show normal C-peptide levels as the disease progresses. C-peptide also contributes to diabetic complications through associations with proteins involved in vascular function suggesting its possible role in conditions like diabetic nephropathy and retinopathy.
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6 product images
Sandwich ELISA - Human C-Peptide ELISA Kit (ab260064)
Human C-peptide standard curve comparison.
Standard Curve comparison between human C-Peptide SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human C-Peptide ELISA Kit (ab260064)
Example of human C-Peptide standard curve in Sample Diluent NS.
The C-Peptide standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human C-Peptide ELISA Kit (ab260064)
Interpolated concentrations of native C-Peptide in human serum, plasma (heparin) and pooled-sex urine samples.
The concentrations of C-Peptide were measured in duplicates, interpolated from the C-Peptide standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (heparin) 12.5%, and urine 1: 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C-Peptide concentration was determined to be 1.3 ng/mL in serum, 0.6 ng/mL in plasma (heparin), and 31.7 ng/mL in pooled-sex urine.
Sandwich ELISA - Human C-Peptide ELISA Kit (ab260064)
Serum from three individual human male donors with type 2 diabetes was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C-Peptide concentration in type 2 diabetes serum samples was determined to be 1.9 ng/mL with a range of 1.4 – 2.2 ng/mL.
Sandwich ELISA - Human C-Peptide ELISA Kit (ab260064)
Interpolated concentration of native C-Peptide was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 12.5% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human C-Peptide ELISA Kit (ab260064)
Example of human C-Peptide standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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