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AB325367

Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent

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Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human C-Peptide (Insulin C-peptide) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :0.46 - 1000 pg/mL with a sensitivity of 0.34 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent Pending

View Alternative Names

Insulin

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Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Dynamic range comparison between the Chemiluminescent Human C-Peptide SimpleStep ELISA, colorimetric Human C-Peptide SimpleStep ELISA (ab260064), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Interpolated concentration of native C-Peptide was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 12.5%, plasma (heparin) 12.5%, and urine 1 : 200. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Serum of eight individual healthy human male donors and serum of four congestive heart failure (CHF) donors was measured in duplicate. Average interpolated dilution factor corrected values (to neat sample) are plotted. The mean C-Peptide concentration of CHF serum was determined to be 5,450 pg/mL with a range of 1,892 – 7,335 pg/mL. The mean C-Peptide concentration of healthy donors was determined to be 669 pg/mL with a range of 17 – 1,467 pg/mL.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean C-Peptide concentration was determined to be 669 pg/mL with a range of 17 – 1,467 pg/mL.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Serum from three individual human male donors with type 2 diabetes was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C-Peptide concentration in type 2 diabetes serum samples was determined to be 1.9 ng/mL with a range of 1.4 – 2.2 ng/mL.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Standard curve comparison between the Chemiluminescent Human C-Peptide SimpleStep ELISA and colorimetric Human C-Peptide SimpleStep ELISA (ab260064) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Serum, Heparin Plasma, Cell culture media, Urine

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.34 pg/mL

Range

0.46 - 1000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (ab325367) is a rapid single-wash sandwich 55-min ELISA to measure Human C-Peptide (Insulin C-peptide) in Serum, Heparin Plasma, Cell Culture Media, Urine with a sensitivity of 0.34 pg/ml.

How the assay works

Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (ab325367) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human C-Peptide (Insulin C-peptide) (ab325367) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Rabbit monoclonal [EPR22500-5] to - BSA and Azide free (Capture) ab259549

- Rabbit monoclonal [EPR20432-228] to human C peptide - BSA and Azide free (Detector) ab281859

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Serum", "range": "100 - 110 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "85 - 102 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "88 - 92 %", "average": "= 90" }, { "sample": "Urine", "range": "101 - 107 %", "average": "= 103" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information INS
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com