Human C1QA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human C1QA in Cell culture media, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.
Colorimetric
Cell culture media, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
Sandwich (quantitative)
Human
125 - 8000 pg/mL
1h 30m
= 27.522 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Complement C1q subcomponent subunit A, C1QA
Human C1QA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human C1QA in Cell culture media, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.
Colorimetric
Cell culture media, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
Sandwich (quantitative)
Human
125 - 8000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 27.522 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.3 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 6.3 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture media | Average % = 96 | Range 84 - 103 % |
Sample type Citrate plasma | Average % = 106 | Range 104 - 107 % |
Sample type EDTA Plasma | Average % = 85 | Range 82 - 87 % |
Sample type Heparin Plasma | Average % = 98 | Range 93 - 107 % |
Sample type Serum | Average % = 94 | Range 83 - 103 % |
Blue Ice
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This supplementary information is collated from multiple sources and compiled automatically.
The C1QA protein is one part of the complement component 1q (C1q) molecule also called C1Q-A. This protein plays an important role in the immune system. The C1q complex consists of three polypeptide chains: C1QA C1QB and C1QC. C1QA alone has a mass of around 26 kDa. These proteins mainly express in immune cells like macrophages dendritic cells and B cells. It forms part of the extracellular matrix where it participates in immune functions.
C1QA interacts with pathogens and modified host cells to trigger the complement cascade. This starts the activation of the classical pathway which enhances immune responses. C1QA together with C1QB and C1QC binds to Fc regions of antibodies attached to antigens. This binding leads to the subsequent activation of C1r and C1s proteases facilitating opsonization and clearance of immune complexes and apoptotic cells.
The synthesis of C1QA plays an important role in the classical complement pathway and is part of the innate immunity. C1QA collaborates with proteins like C1QB and C1QC in this pathway. This pathway bridges innate and adaptive immunity by assisting in the removal of pathogens and cell debris. It also relates to the coagulation pathway where its activity can influence inflammation and tissue repair processes.
Impaired C1QA function links to conditions like systemic lupus erythematosus (SLE) and rheumatoid arthritis. These diseases often associate with inappropriate activation or regulation of the complement system. C1QA mutations or deficiencies can result in a higher susceptibility to SLE and interactions with C1QB and C1QC are also implicated in this disease. Moreover C1QA influences the pathogenesis of rheumatoid arthritis by modulating inflammation responses.
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Example of human C1QA standard curve in Sample Diluent NS.
Example of human C1QA standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of C1QA in normal human serum donors.
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean C1QA concentration was determined to be 105.6 μg/mL with a range of 61.8 – 138.2 μg/mL.
Interpolated concentrations of human C1QA in serum, plasma-citrate, plasma-EDTA, and plasma-heparin.
Interpolated concentration of native C1QA was measured in duplicate at different sample concentrations. Undiluted samples are 1:10,000 in serum, plasma-citrate, plasma-EDTA, and plasma-heparin. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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