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Human C1QA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human C1QA in Cell culture media, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.

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Images

Sandwich ELISA - Human C1QA ELISA Kit (AB300309), expandable thumbnail
  • Sandwich ELISA - Human C1QA ELISA Kit (AB300309), expandable thumbnail
  • Sandwich ELISA - Human C1QA ELISA Kit (AB300309), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

125 - 8000 pg/mL

Assay time

1h 30m

Sensitivity

= 27.522 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Alternative names

What's included?

96 Tests
Components
10X Human C1QA Capture Antibody
1 x 600 µL
10X Human C1QA Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Denaturant
1 x 500 µL
Human C1QA Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human C1QA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human C1QA in Cell culture media, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 27.522 pg/mL

Precision

Intra assay

Sample

Serum

n
8
mean
-
SD
-
C.V.

5.3

Inter assay

Sample

Serum

n
3
mean
-
SD
-
C.V.

6.3

Recovery

Sample specific recovery

Sample type

Cell culture media

Average %

= 96

Range

84 - 103 %

Sample type

Citrate plasma

Average %

= 106

Range

104 - 107 %

Sample type

EDTA Plasma

Average %

= 85

Range

82 - 87 %

Sample type

Heparin Plasma

Average %

= 98

Range

93 - 107 %

Sample type

Serum

Average %

= 94

Range

83 - 103 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The C1QA protein is one part of the complement component 1q (C1q) molecule also called C1Q-A. This protein plays an important role in the immune system. The C1q complex consists of three polypeptide chains: C1QA C1QB and C1QC. C1QA alone has a mass of around 26 kDa. These proteins mainly express in immune cells like macrophages dendritic cells and B cells. It forms part of the extracellular matrix where it participates in immune functions.

Biological function summary

C1QA interacts with pathogens and modified host cells to trigger the complement cascade. This starts the activation of the classical pathway which enhances immune responses. C1QA together with C1QB and C1QC binds to Fc regions of antibodies attached to antigens. This binding leads to the subsequent activation of C1r and C1s proteases facilitating opsonization and clearance of immune complexes and apoptotic cells.

Pathways

The synthesis of C1QA plays an important role in the classical complement pathway and is part of the innate immunity. C1QA collaborates with proteins like C1QB and C1QC in this pathway. This pathway bridges innate and adaptive immunity by assisting in the removal of pathogens and cell debris. It also relates to the coagulation pathway where its activity can influence inflammation and tissue repair processes.

Associated diseases and disorders

Impaired C1QA function links to conditions like systemic lupus erythematosus (SLE) and rheumatoid arthritis. These diseases often associate with inappropriate activation or regulation of the complement system. C1QA mutations or deficiencies can result in a higher susceptibility to SLE and interactions with C1QB and C1QC are also implicated in this disease. Moreover C1QA influences the pathogenesis of rheumatoid arthritis by modulating inflammation responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Human C1QA ELISA Kit (ab300309), expandable thumbnail

    Sandwich ELISA - Human C1QA ELISA Kit (ab300309)

    Example of human C1QA standard curve in Sample Diluent NS.

    Example of human C1QA standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human C1QA ELISA Kit (ab300309), expandable thumbnail

    Sandwich ELISA - Human C1QA ELISA Kit (ab300309)

    Interpolated concentrations of C1QA in normal human serum donors.

    Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean C1QA concentration was determined to be 105.6 μg/mL with a range of 61.8 – 138.2 μg/mL.

  • Sandwich ELISA - Human C1QA ELISA Kit (ab300309), expandable thumbnail

    Sandwich ELISA - Human C1QA ELISA Kit (ab300309)

    Interpolated concentrations of human C1QA in serum, plasma-citrate, plasma-EDTA, and plasma-heparin.

    Interpolated concentration of native C1QA was measured in duplicate at different sample concentrations. Undiluted samples are 1:10,000 in serum, plasma-citrate, plasma-EDTA, and plasma-heparin. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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