AB323699

Human C3a anaphylatoxin ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human C3a anaphylatoxin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human C3a anaphylatoxin with a sensitivity of 1.745 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

6 Images

Sandwich ELISA - Human C3a anaphylatoxin ELISA Kit (AB323699)

sELISA

Supplier Data

Sandwich ELISA - Human C3a anaphylatoxin ELISA Kit (AB323699)

Example of human C3a anaphylatoxin standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human C3a anaphylatoxin ELISA Kit (AB323699)

Interpolated concentration of native C3a anaphylatoxin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 cell culture supernatant 1 : 40. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human C3a anaphylatoxin ELISA Kit (AB323699)

Interpolated concentration of native C3a anaphylatoxin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 50,000, plasma – citrate 1 : 100,000, plasma – EDTA 1 : 50,000, and plasma – heparin 1 : 50,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human C3a anaphylatoxin ELISA Kit (AB323699)

Interpolated concentration of native C3a anaphylatoxin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 80 HepG2 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human C3a anaphylatoxin ELISA Kit (AB323699)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean C3a anaphylatoxin concentration was determined to be 3.50 µg/mL with a range of 0.38 – 8.93 µg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human C3a anaphylatoxin ELISA Kit (AB323699)

Example of human C3a anaphylatoxin standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.745 pg/mL

Range

3.906 - 250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human C3a anaphylatoxin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C3a anaphylatoxin protein in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant. Quantitate Human C3a anaphylatoxin with 1.745 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.4" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "96 - 102 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "91 - 97 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "94 - 103 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "97 - 107 %", "average": "= 101" }, { "sample": "Cell culture supernatant", "range": "100 - 116 %", "average": "= 109" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab323699-10x96Tests", "assetComponentDetails": [ { "size":"20 x 1 Vial", "name":"Human C3a anaphylatoxin Lyophilized Recombinant Protein", "number":"AB323699-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human C3a anaphylatoxin Detector Antibody", "number":"AB323699-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human C3a anaphylatoxin Capture Antibody", "number":"AB323699-CMP01" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB323699-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323699-CMP06" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323699-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323699-CMP05" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB323699-CMP07" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB323699-CMP10" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323699-CMP04" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab323699-1x384Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human C3a anaphylatoxin Lyophilized Recombinant Protein", "number":"AB323699-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human C3a anaphylatoxin Detector Antibody", "number":"AB323699-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human C3a anaphylatoxin Capture Antibody", "number":"AB323699-CMP01" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB323699-CMP08" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB323699-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323699-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB323699-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323699-CMP10" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323699-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323699-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab323699-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB323699-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human C3a anaphylatoxin Lyophilized Recombinant Protein", "number":"AB323699-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human C3a anaphylatoxin Detector Antibody", "number":"AB323699-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human C3a anaphylatoxin Capture Antibody", "number":"AB323699-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323699-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323699-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323699-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323699-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB323699-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323699-CMP10", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Precursor of non-enzymatic components of the classical, alternative, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.. Complement C3b. Non-enzymatic component of C5 convertase (PubMed : 28264884, PubMed : 31507604, PubMed : 3653927, PubMed : 3897448). Generated following cleavage by C3 convertase, it covalently attaches to the surface of pathogens, where it acts as an opsonin that marks the surface of antigens for removal (PubMed : 28264884, PubMed : 31507604, PubMed : 3653927, PubMed : 3897448, PubMed : 833545, PubMed : 8349625). Complement C3b binds covalently via its reactive thioester, to cell surface carbohydrates or immune aggregates (PubMed : 6903192). Together with complement C4b, it then recruits the serine protease complement C2b to form the C5 convertase, which cleaves and activate C5, the next component of the complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 2387864). In the alternative complement pathway, recruits the serine protease CFB to form the C5 convertase that cleaves and activates C5 (PubMed : 624565, PubMed : 6554279).. C3a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C3 convertase (PubMed : 6968751, PubMed : 37169960, PubMed : 37852260). Acts by binding to its receptor, C3AR1, activating G protein-coupled receptor signaling, promoting the phosphorylation, ARRB2-mediated internalization and endocytosis of C3AR1 (PubMed : 8702752, PubMed : 37169960, PubMed : 37852260). C3a anaphylatoxin stimulates the activation of immune cells such as mast cells and basophilic leukocytes to release inflammation agents, such as cytokines, chemokines and histamine, which promote inflammation development (PubMed : 23383423). Also acts as potent chemoattractant for the migration of macrophages and neutrophils to the inflamed tissues, resulting in neutralization of the inflammatory triggers by multiple ways, such as phagocytosis and generation of reactive oxidants (PubMed : 23383423, PubMed : 342601, PubMed : 5778786).. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial triglyceride clearance (PubMed : 10432298, PubMed : 15833747, PubMed : 16333141, PubMed : 19615750, PubMed : 2909530, PubMed : 8376604, PubMed : 9059512). Appears to stimulate triglyceride synthesis via activation of the PLC, MAPK and AKT signaling pathways (PubMed : 16333141). Acts by binding to its receptor, C5AR2, activating G protein-coupled receptor signaling, promoting the phosphorylation, ARRB2-mediated internalization and endocytosis of C5AR2 (PubMed : 11773063, PubMed : 12540846, PubMed : 19615750). In contrast to C3a anaphylatoxin peptide, does not show pro-inflammatory activity (PubMed : 37852260).. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.

See full target information C3

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com