AB279352
Human C3a ELISA kit is a sandwich ELISA designed to quantify Human C3a with a sensitivity of 63 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 62.5 - 4000 pg/mL
View Alternative Names
CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human C3a ELISA kit (AB279352)
These standard curves are for demonstration only. A standard curve must be run with each assay.
Complementary Products
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human C3a ELISA kit ab279352 is a sandwich ELISA to measure Human C3a in serum, plasma, cell culture supernatant with a sensitivity of 63 pg/ml.
How the assay works
This assay employs an antibody specific for human C3a coated on a 96-well plate. Standards and samples are pipetted into the wells and C3a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human C3a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of C3a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human C3a ELISA kit ab279352 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
How the assay works
This assay employs an antibody specific for human C3a coated on a 96-well plate. Standards and samples are pipetted into the wells and C3a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human C3a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of C3a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human C3a ELISA kit ab279352 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
Precision
[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
Recovery
[ { "sample": "Serum", "range": "80 - 105 %", "average": "= 96.46" }, { "sample": "Plasma", "range": "86 - 105 %", "average": "= 97.3" }, { "sample": "Cell culture media", "range": "85 - 100 %", "average": "= 94.88" } ]
What's included?
{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab279352-1Kit", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279352-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB279352-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279352-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-human C3a coated microplate", "number":"AB279352-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-human C3a antibody", "number":"AB279352-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human C3a Standard Protein (Lyophilized)", "number":"AB279352-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279352-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279352-CMP02", "productcode":"" } ] } } }
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
The Complement Component 3 (C3) is a central protein in the complement system often referred to as C3 or C3a when discussing its active fragment. C3 weighs approximately 187 kDa and expresses widely in the liver then circulating in blood plasma. When activated C3 cleaves into C3a and C3b. C3a acts as an anaphylatoxin contributing to the immune response by promoting inflammation. C3 plays critical roles in immunity by enhancing phagocytosis and clearance of pathogens.
Biological function summary
C3 fragments particularly C3a influence various immune responses. As part of the complement system C3 integrates into a complex network of proteins. This activation cascade enhances the body's ability to combat pathogens. C3a interacts with receptors on immune cells mediating inflammatory response and acting as a chemoattractant. C3b another key fragment binds to pathogen surfaces aiding opsonization and immune clearance.
Pathways
C3's function plays an important role in the alternative and classical complement pathways. In the alternative pathway C3b contributes directly by forming a C3 convertase complex. This interaction facilitates further cleavage of C3 amplifying immune response. C3b also associates with proteins like Factor B and Factor D to stabilize the convertase complex and enhance opsonization. In the classical pathway C3 interacts with components like C4b to optimize pathogen elimination.
C3 and its fragments have significant connections to immune disorders and infections. Low levels or dysfunctional C3 can lead to susceptibility to bacterial infections. C3a has associations with asthma where it exacerbates the inflammatory response in lung tissues. In autoimmune diseases such as systemic lupus erythematosus (SLE) improper regulation of C3 activation leads to tissue damage. The interaction of C3 with proteins like C1q in SLE highlights the interplay between complement activation and autoimmune pathologies.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Precursor of non-enzymatic components of the classical, alternative, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.. Complement C3b. Non-enzymatic component of C5 convertase (PubMed : 28264884, PubMed : 31507604, PubMed : 3653927, PubMed : 3897448). Generated following cleavage by C3 convertase, it covalently attaches to the surface of pathogens, where it acts as an opsonin that marks the surface of antigens for removal (PubMed : 28264884, PubMed : 31507604, PubMed : 3653927, PubMed : 3897448, PubMed : 833545, PubMed : 8349625). Complement C3b binds covalently via its reactive thioester, to cell surface carbohydrates or immune aggregates (PubMed : 6903192). Together with complement C4b, it then recruits the serine protease complement C2b to form the C5 convertase, which cleaves and activate C5, the next component of the complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 2387864). In the alternative complement pathway, recruits the serine protease CFB to form the C5 convertase that cleaves and activates C5 (PubMed : 624565, PubMed : 6554279).. C3a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C3 convertase (PubMed : 6968751, PubMed : 37169960, PubMed : 37852260). Acts by binding to its receptor, C3AR1, activating G protein-coupled receptor signaling, promoting the phosphorylation, ARRB2-mediated internalization and endocytosis of C3AR1 (PubMed : 8702752, PubMed : 37169960, PubMed : 37852260). C3a anaphylatoxin stimulates the activation of immune cells such as mast cells and basophilic leukocytes to release inflammation agents, such as cytokines, chemokines and histamine, which promote inflammation development (PubMed : 23383423). Also acts as potent chemoattractant for the migration of macrophages and neutrophils to the inflamed tissues, resulting in neutralization of the inflammatory triggers by multiple ways, such as phagocytosis and generation of reactive oxidants (PubMed : 23383423, PubMed : 342601, PubMed : 5778786).. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial triglyceride clearance (PubMed : 10432298, PubMed : 15833747, PubMed : 16333141, PubMed : 19615750, PubMed : 2909530, PubMed : 8376604, PubMed : 9059512). Appears to stimulate triglyceride synthesis via activation of the PLC, MAPK and AKT signaling pathways (PubMed : 16333141). Acts by binding to its receptor, C5AR2, activating G protein-coupled receptor signaling, promoting the phosphorylation, ARRB2-mediated internalization and endocytosis of C5AR2 (PubMed : 11773063, PubMed : 12540846, PubMed : 19615750). In contrast to C3a anaphylatoxin peptide, does not show pro-inflammatory activity (PubMed : 37852260).. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
Cells 12: PubMed37174708
2023
Exploring the Therapeutic Potential of Elastase Inhibition in Age-Related Macular Degeneration in Mouse and Human.
Applications
Unspecified application
Species
Unspecified reactive species
Soumya Navneet,Carlene Brandon,Kit Simpson,Bärbel Rohrer
Polymers 14: PubMed35890719
2022
Hemocompatibility Evaluation of Thai Silk Fibroin and Its Improvement with Low Molecular Weight Heparin Immobilization.
Applications
Unspecified application
Species
Unspecified reactive species
Tanrada Fungmongkonsatean,Jirapas Jongjitwimol,Pussadee Paensuwan,Teonchit Nuamchit,Duangduan Siriwittayawan,Sorada Kanokpanont,Siriporn Damrongsakkul,Piyanuch Thitiwuthikiat
Product promise
We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com