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AB315320

Human C4a anaphylatoxin ELISA Kit

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Human C4a anaphylatoxin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human C4a anaphylatoxin with a sensitivity of 12.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

CO4, CPAMD2, C4A, Complement C4-A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2

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Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)
  • sELISA

Supplier Data

Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)

Interpolated concentrations of human C4a anaphylatoxin in human plasma – heparin and milk. Interpolated concentration of native C4a anaphylatoxin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human plasma – heparin 1 : 1,600 and human milk 1 : 67.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)
  • sELISA

Supplier Data

Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)

Interpolated concentrations of human C4a anaphylatoxin in human serum, plasma – citrate, and plasma – EDTA. Interpolated concentration of native C4a anaphylatoxin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 1 : 10,000, human plasma – citrate 1 : 5,000, and human plasma – EDTA 1 : 6,400. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)
  • sELISA

Supplier Data

Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)

Interpolated concentrations of C4a anaphylatoxin in normal human serum donors. Serum of seven individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C4a anaphylatoxin concentration was determined to be 4,579 ng/mL with a range of 991 – 7,804 ng/mL.

Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)
  • sELISA

Lab

Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)

Example of human C4a anaphylatoxin standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)
  • sELISA

Supplier Data

Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)

Representative image of Human C4a anaphylatoxin ELISA Kit (ab315320)

Components shown from left to right :

- Sample diluent NS (2 bottles)

- 10X Human C4a anaphylatoxin Detector Antibody

- Human C4a anaphylatoxin Lyophilised Purified Protein (2 vials)

- Human C4a anaphylatoxin Capture Antibody (Lyophilised)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Milk

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 12.7 pg/mL

Range

37.5 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human C4a anaphylatoxin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C4a anaphylatoxin protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, and Milk. Quantitate Human C4a anaphylatoxin with 4.245 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.9" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 108 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "100 - 115 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "100 - 108 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "85 - 103 %", "average": "= 92" }, { "sample": "Milk", "range": "101 - 106 %", "average": "= 104" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315320-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Lyophilized Human C4a anaphylatoxin Capture Antibody", "number":"AB315320-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human C4a anaphylatoxin Detector Antibody", "number":"AB315320-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human C4a anaphylatoxin Lyophilized Recombinant Protein", "number":"AB315320-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB315320-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315320-CMP05", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315320-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315320-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315320-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315320-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315320-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement C4a also known as C4a anaphylatoxin is a component of the complement system with a molecular mass of approximately 9 kDa. This small protein originates from the proteolytic cleavage of complement component C4 which contributes to immune system activation. C4a is mainly expressed in the liver but can also be produced by other tissues during inflammation. It serves as a chemoattractant recruiting immune cells to sites of infection helping initiate a response against pathogens.
Biological function summary

Complement C4a acts as an anaphylatoxin participating in the complex immune system response. Anaphylatoxins like C4a C3a and C5a contribute to the inflammatory process by inducing smooth muscle contraction increasing vascular permeability and triggering histamine release from mast cells and basophils. C4a complements the activities of other complement components playing an important role in modulating inflammation and defense mechanisms in the body.

Pathways

The activation of C4a is an important step within the classical and lectin pathways of complement activation. These pathways involve the recognition and elimination of pathogens and unwanted cells. C4a along with its related proteins C4b and components C2 and C3 facilitates the formation of C3 convertase which amplifies the response and further clears pathogens. C4a also interacts with complement inhibitors which regulate these pathways to prevent excessive tissue damage during inflammation.

C4a anaphylatoxin has associations with autoimmune diseases and angioneoplasm. Changes in serum levels of C4a are observed in conditions like systemic lupus erythematosus where it may contribute to tissue damage. High C4a levels can correlate with disease activity and severity. Additionally C4a alongside other anaphylatoxins like C3a and C5a might play a role in angiogenesis influencing tumor progression and angiogenesis-related disorders serving as a potential biomarker for disease severity and prognosis.
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Product protocols

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Target data

Precursor of non-enzymatic components of the classical, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.. Complement C4b-A. Non-enzymatic component of C3 and C5 convertases (PubMed : 8538770). Generated following cleavage by complement proteases (C1S, MASP2 or GZMK, depending on the complement pathway), it covalently attaches to the surface of pathogens, where it acts as an opsonin that marks the surface of antigens for removal (PubMed : 27738201, PubMed : 8538770). It then recruits the serine protease complement C2b to form the C3 and C5 convertases, which cleave and activate C3 and C5, respectively, the next components of the complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 2387864, PubMed : 6906228). Complement C4b-A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while complement C4b-B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens (PubMed : 8538770).. C4a anaphylatoxin. Putative humoral mediator released following cleavage by complement proteases (C1S, MASP2 or GZMK, depending on the complement pathway) (PubMed : 6167582). While it is strongly similar to anaphylatoxins, its role is unclear (PubMed : 25659340). Was reported to act as a mediator of local inflammatory process; however these effects were probably due to contamination with C3a and/C5a anaphylatoxins in biological assays (PubMed : 25659340).
See full target information C4A
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