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AB313519

Human C4c ELISA Kit

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Human C4c ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human C4c in Human in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Human C4c ELISA Kit (AB313519)
  • sELISA

Supplier Data

Sandwich ELISA - Human C4c ELISA Kit (AB313519)

Example of standard curve using ab313519. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 21 ng/mL

Range

21 - 5000 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human C4c ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human C4c in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human C4c coated on a 96-well plate.
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Recovery

[ { "sample": "Cell culture media", "range": "71 - 126 %", "average": "= 90.47" }, { "sample": "Plasma", "range": "68 - 110 %", "average": "= 83.56" }, { "sample": "Serum", "range": "68 - 110 %", "average": "= 76.74" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313519-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human C4c Protein Standard", "number":"AB313519-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313519-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human C4c Detection Antibody", "number":"AB313519-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313519-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB313519-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human C4c Antibody-coated ELISA Plate", "number":"AB313519-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313519-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313519-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement C4 also known as C4 or C4A/C4B plays an important role in the immune system. It is a component of the classical complement pathway. The protein has a relative molecular mass of approximately 210 kDa. Complement C4 is primarily expressed in the liver and is then released into the bloodstream. Through interactions with other complement proteins C4 acts as a critical effector molecule in the immune response promoting the opsonization of pathogens.
Biological function summary

The role of Complement C4 involves the formation of the C3 convertase complex which amplifies the immune response. This protein participates in the clearance of immune complexes and apoptotic cells. By binding to pathogens C4 assists in their opsonization and subsequent phagocytosis by immune cells. Complement C4 is a part of the larger complement system a critical component of innate immunity that facilitates the elimination of invading microbes.

Pathways

Complement C4 is central to the classical and lectin pathways of complement activation. In the classical pathway the protein works with Complement C1 and C2 to form the C3 convertase which cleaves and activates Complement C3 setting off a cascade that generates the membrane attack complex. Additionally in the lectin pathway C4 contributes similarly to amplification of immune responses highlighting its adaptability within various cascades.

Mutations or deficiencies in Complement C4 have associations with autoimmune diseases like systemic lupus erythematosus (SLE) and hereditary angioedema. People with C4 deficiencies experience increased susceptibility to recurring infections and autoimmune conditions and the protein often connects with other complements like C2 and C3 during disease pathophysiology. Understanding the function and dysfunction of Complement C4 helps in the diagnosis and treatment of these disorders.
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Product protocols

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Target data

See full target information C4C
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websiteProtocolBooklet
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