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AB304938

Human C7 ELISA Kit

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Human C7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human C7 with a sensitivity of 18.846 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Complement component C7, C7

3 Images
Sandwich ELISA - Human C7 ELISA Kit (AB304938)
  • sELISA

Supplier Data

Sandwich ELISA - Human C7 ELISA Kit (AB304938)

Serum of four individual healthy human female donors and four individual healthy Human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean C7 concentration was determined to be 42.6 µg/mL with a range of 29.0 - 55.4 µg/mL.

Sandwich ELISA - Human C7 ELISA Kit (AB304938)
  • sELISA

Supplier Data

Sandwich ELISA - Human C7 ELISA Kit (AB304938)

Example of human C7 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human C7 ELISA Kit (AB304938)
  • sELISA

Supplier Data

Sandwich ELISA - Human C7 ELISA Kit (AB304938)

Interpolated concentration of native C7 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 10,000 serum, plasma (EDTA), plasma (heparin), and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 18.846 pg/mL

Range

58.594 - 3750 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human C7 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C7 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human C7 with 18.846 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.52" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "89 - 101 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "103 - 124 %", "average": "= 113" }, { "sample": "EDTA Plasma", "range": "102 - 109 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "86 - 103 %", "average": "= 94" }, { "sample": "Serum", "range": "93 - 104 %", "average": "= 98" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 7 (C7) with a molecular mass of approximately 93 kDa plays an essential mechanical role in the complement system a critical part of innate immunity. C7 is one of the late components of the complement cascade involved in the formation of the membrane attack complex (MAC). It is primarily expressed in the liver and found in the blood plasma. By associating with other components C5b and C6 C7 anchors into the lipid bilayer of target cells serving as a foothold for further MAC assembly.
Biological function summary

C7 functions as an important facilitator in the innate immune response being an integral part of the membrane attack complex (MAC). This complex forms transmembrane channels in the cell membrane of pathogens leading to their lysis and destruction. Through its interaction with complement components like C5b and C6 C7 enables the insertion and stabilization of the MAC. This process is important for the immune system’s ability to eliminate pathogens quickly and effectively protecting the host from microbial invaders.

Pathways

The complement system pathway is the primary biological pathway involving C7. Within this pathway C7 interacts closely with complement proteins C5 and C6 contributing to the terminal pathway that leads to pathogen lysis. Besides it is linked with the classical and alternative complement pathways both of which converge at the activation of C5. Through these interactions and processes C7 works synergistically with other complement proteins to execute robust immune defense mechanisms.

C7 deficiencies have been associated with an increased susceptibility to infections particularly by Neisseria species due to impaired MAC formation. This link highlights the importance of C7 for adequate host defense. Additionally overactivation of the complement system potentially involving C7 contributes to the pathology of autoimmune disorders like systemic lupus erythematosus. In such conditions dysregulation involving proteins like C5b and C6 can lead to tissue damage and inflammation further illustrating the importance of C7 in maintaining immune homeostasis.
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Product protocols

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Target data

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.
See full target information C7
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websiteProtocolBooklet
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