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AB288173

Human C8a (Complement C8a) ELISA Kit

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Human C8a (Complement C8a) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human C8a (Complement C8a) with a sensitivity of 40.141 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Complement component C8 alpha chain, Complement component 8 subunit alpha, C8A

4 Images
Sandwich ELISA - Human C8a (Complement C8a) ELISA Kit (AB288173)
  • sELISA

Supplier Data

Sandwich ELISA - Human C8a (Complement C8a) ELISA Kit (AB288173)

Interpolated concentrations of C8a in normal human serum donors.

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean C8a concentration was determined to be 55.3 ug/mL with a range of 38.35 - 73.45 ug/mL.

Sandwich ELISA - Human C8a (Complement C8a) ELISA Kit (AB288173)
  • sELISA

Supplier Data

Sandwich ELISA - Human C8a (Complement C8a) ELISA Kit (AB288173)

Interpolated concentrations of human C8a in serum, plasma (EDTA), plasma (citrate), and plasma (heparin). Interpolated concentration of native C8a was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 8,000, plasma (EDTA)1 : 8,000, plasma (citrate)1 : 8,000, and plasma (heparin)1 : 4,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human C8a (Complement C8a) ELISA Kit (AB288173)
  • sELISA

Supplier Data

Sandwich ELISA - Human C8a (Complement C8a) ELISA Kit (AB288173)

Interpolated concentrations of human C8a in milk and saliva. Interpolated concentration of native C8a was measured in duplicate at different sample concentrations. Undiluted samples are as follows : milk 5% and saliva 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human C8a (Complement C8a) ELISA Kit (AB288173)
  • sELISA

Supplier Data

Sandwich ELISA - Human C8a (Complement C8a) ELISA Kit (AB288173)

Example of human C8a standard curve in Sample Diluent NS. Example of human C8a standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Cell culture media, Heparin Plasma, Citrate plasma, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 40.141 pg/mL

Range

85.938 - 5500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human C8a (Complement C8a) ELISA Kit (ab288172) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Complement C8a protein in cell culture supernatant, plasma, milk, saliva and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Complement C8a with 40.141 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]
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Recovery

[ { "sample": "Milk", "range": "84 - 100 %", "average": "= 91" }, { "sample": "Serum", "range": "91 - 109 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "82 - 108 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "96 - 106 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "94 - 109 %", "average": "= 100" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement C8a also known as C8 alpha chain is a part of the complement system which plays a role in immune response. It has a molecular mass of approximately 64 kDa. This protein is found in blood plasma and is mainly expressed in hepatocytes. The C8a forms the terminal subunit of the C8 protein complex which is critical for the formation of the membrane attack complex (MAC) that disrupts the lipid bilayer of target cells.
Biological function summary

The C8a chain works in concert with C8 beta and C8 gamma to form the C8 complex of the complement system. This protein becomes part of the larger MAC a lytic complex that facilitates cell membrane disruptions thereby initiating cell lysis. The membrane attack complex plays a defensive role against pathogenic microorganisms contributing to innate immunity.

Pathways

The complement system involving C8a plays a central role in the innate immune pathway and the alternative pathway of complement activation. These pathways are essential for the protection against infections. The C5 convertase acts upstream of C8a cleaving C5 to C5b which then associates with C6 and C7 allowing C8a and its counterparts C8b and C8g to bind and initiate MAC formation. C9 is the final protein to bind completing the complex and resulting in the lysis of the target.

The deficiency or dysfunction in C8a can be associated with increased susceptibility to Neisseria infections particularly meningococcal disease. C8 deficiency is often linked with deficiencies in C5-C9 proteins as these proteins are collectively critical for functional MAC formation. Immunological responses depending on the formation of the MAC can become compromised increasing vulnerability to certain bacterial infections.
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Product protocols

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Target data

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.
See full target information C8A
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websiteProtocolBooklet
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