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AB287178

Human C9 ELISA Kit (Complement C9)

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Human C9 ELISA Kit (Complement C9) is a single-wash 90-min Simplestep used to quantify Human C9 (Complement C9) with a sensitivity of 10.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Complement component C9, C9

4 Images
Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)
  • sELISA

Supplier Data

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Interpolated concentrations of human C9 in serum, plasma (heparin), plasma (EDTA), and plasma (citrate).

Interpolated concentration of native C9 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 20,000 serum, plasma (heparin), plasma (EDTA), and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)
  • sELISA

Supplier Data

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Interpolated concentrations of human C9 in milk.

Interpolated concentration of native C9 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 167 milk. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)
  • sELISA

Supplier Data

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Interpolated concentrations of C9 in normal human serum donors.

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean C9 concentration was determined to be 73.8 μg/mL with a range of 28.5 – 99.3 μg/mL.

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)
  • sELISA

Supplier Data

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Example of human C9 standard curve in Sample Diluent NS.

Example of human C9 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 10.6 pg/mL

Range

43.75 - 2800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human C9 ELISA kit (ab287178) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human C9 protein in human serum, plasma, and milk samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human C9 with 10.6 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" } ]
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Recovery

[ { "sample": "Milk", "range": "101 - 102 %", "average": "= 102" }, { "sample": "Serum", "range": "118 - 119 %", "average": "= 118" }, { "sample": "EDTA Plasma", "range": "110 - 117 %", "average": "= 114" }, { "sample": "Heparin Plasma", "range": "103 - 112 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "115 - 118 %", "average": "= 116" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C9 also known as complement component 9 is a protein that plays an essential mechanical role in the complement system. It typically associates in the formation of the membrane attack complex (MAC). The C9 protein weighing around 71 kDa expresses in the liver and is secreted into the blood plasma. Alternative names like 'Cloud 9 capture' and 'C9 tag' often refer to research tools or motifs derived from the C9 protein rather than the protein itself. Researchers frequently study C9 in mouse models where its physiological roles can be explored.
Biological function summary

C9 participates in defending against pathogens. It joins other complement components like C5b C6 C7 and C8 to form the MAC complex which facilitates cell lysis by forming pores in the membrane of target cells. This action directly results in microbial cell destruction serving a critical immune function. C9 is not only part of the MAC complex but also holds significance due to its unique function as the last step in the formation of the pore influencing the efficacy of pathogen elimination.

Pathways

C9 functions primarily within the complement cascade which includes the classical and alternative pathways. Its role is in the terminal pathway segment that ultimately leads to the formation of the MAC and follows the activation of C5 which cleaves to produce C5b. This connection extends to proteins like C3 which is upstream in the cascade and significantly impacts the cascade's activation highlighting C9's function as an integral terminal component in these pathways.

Mutations or deficits in C9 relate to an increased susceptibility to infections particularly those caused by Neisseria species. This association results directly from its important role in the MAC formation. Additionally deficiency of C9 links to autoimmune disorders where the immune response may be improperly regulated due to compromised MAC formation. Other complement components like C6 and C8 may also relate to these diseases due to their collaboration with C9 in forming the MAC illustrating the interconnectedness of these proteins within the immune response framework.
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Product protocols

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Target data

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells (PubMed : 26841934, PubMed : 9212048, PubMed : 9634479). C9 is the pore-forming subunit of the MAC (PubMed : 26841934, PubMed : 30111885, PubMed : 4055801).
See full target information C9
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