AB274402

Human CA125 ELISA Kit (MUC16)

Recombinant
SimpleStep
What is this?

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(3 Publications)

Human CA125 ELISA Kit (MUC16) is a single-wash 90-min Simplestep used to quantify Human CA125 (MUC16) with a sensitivity of 0.02 U/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CA125, MUC16, Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, CA-125

6 Images

ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

ELISA

Supplier Data

ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

sELISA

Unknown

Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Serum from eight individual healthy CA125 female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CA125 concentration was determined to be 0.2 U/mL, 6 donors CA125 were below detection level.

sELISA

Supplier Data

Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Human CA125 competitor curve comparison.

Standard Curve comparison between Human CA125 SimpleStep ELISA kit and traditional ELISA kit from leading competitor.

sELISA

Supplier Data

Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Interpolated concentrations of native CA125 in Hela cell culture supernatant and milk samples.

The concentrations of CA125 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : Hela cell culture supernatant, 50%, milk, 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 3 U/mL in Hela cell culture supernatant, 4.5 U/mL in milk.

sELISA

Supplier Data

Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Human CA125 standard curve comparison.

Standard Curve comparison between the original Human CA125 SimpleStep ELISA kit (ab195213) and current Human CA125 SimpleStep ELISA kit (ab274402).

sELISA

Supplier Data

Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Example of human CA125 standard curve in Sample Diluent NS.

The CA125 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.02 U/mL

Range

0.09 - 6 U/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CA125 ELISA Kit (MUC16) ab274402 is a rapid single-wash 90-min Sandwich ELISA to measure Human CA125 (MUC16)in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, serum, urine. This SimpleStep sensitivity is 0.02 0.

How the assay works

Human CA125 (MUC16)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CA125 ELISA Kit (MUC16) ab274402 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Cancer antigen 125 (CA125, also known as Mucin 16) is a membrane associated cell surface protein containing approximately 22,096 amino acids. CA125 is the largest member of the Mucin family of proteins and is highly O-glycosylated. The hydrophilic nature of mucin proteins gives them the ability to form protective/lubricating gel-like barriers to protect against chemotherapy agents, foreign particles and infectious agents. CA125 is used as a biomarker for ovarian cancer detection, with about 90% of women with advanced ovarian cancer having elevated levels of CA125 present in their blood serum.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "105 - 119 %", "average": "= 110" }, { "sample": "Milk", "range": "92 - 97 %", "average": "= 94" }, { "sample": "Serum", "range": "103 - 105 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "100 - 107 %", "average": "= 103" }, { "sample": "Urine", "range": "90 - 91 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "100 - 107 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "92 - 97 %", "average": "= 94" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

See full target information MUC16

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia 46: PubMed39669306

2024

Systemic inflammatory indices as a non-invasive grading modality for endometriosis: a comparative study versus exploratory laparoscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Sabra Ibrahim Mohammed Sabra,Shreen Naguib Aboelezz Moselhy,Ahmed Kasem Mohamed Zain Eldin

Biomedicines 10: PubMed35453583

2022

Urinary Biomarkers for Detection of Clinical Endometriosis or Adenomyosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Chun Chen,Chao-Min Cheng,Wan-Ting Liao,Ting-Chang Chang

Computational and mathematical methods in medicine 2021:9987067 PubMed34257703

2021

Construction and Validation of a Lung Cancer Diagnostic Model Based on 6-Gene Methylation Frequency in Blood, Clinical Features, and Serum Tumor Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Kang,Dandan Wang,Xiuzhi Zhang,Lingxiao Wang,Fengxiang Wang,Jie Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CA125 ELISA Kit (MUC16)

ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

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Product promise