Human CA125 ELISA Kit (MUC16) is a single-wash 90-min Simplestep used to quantify Human CA125 (MUC16) with a sensitivity of 0.02 U/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Biomedicines 10:2022Urinary Biomarkers for Detection of Clinical Endometriosis or Adenomyosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Chun Chen,Chao-Min Cheng,Wan-Ting Liao,Ting-Chang ChangPubMed 35453583
- Computational and mathematical methods in medicine 2021:99870672021Construction and Validation of a Lung Cancer Diagnostic Model Based on 6-Gene Methylation Frequency in Blood, Clinical Features, and Serum Tumor Markers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunyan Kang,Dandan Wang,Xiuzhi Zhang,Lingxiao Wang,Fengxiang Wang,Jie ChenPubMed 34257703
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.09 - 6 U/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.02 U/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Alternative names
CA125, MUC16, Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, CA-125
What's included?
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Human CA125 ELISA Kit (MUC16) is a single-wash 90-min Simplestep used to quantify Human CA125 (MUC16) with a sensitivity of 0.02 U/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.09 - 6 U/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.02 U/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 5.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 4 | mean - | SD - | C.V. 7.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 110 | Range 105 - 119 % |
Sample type Milk | Average % = 94 | Range 92 - 97 % |
Sample type Serum | Average % = 104 | Range 103 - 105 % |
Sample type EDTA Plasma | Average % = 103 | Range 100 - 107 % |
Sample type Urine | Average % = 91 | Range 90 - 91 % |
Sample type Heparin Plasma | Average % = 103 | Range 100 - 107 % |
Sample type Citrate plasma | Average % = 94 | Range 92 - 97 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CA125 ELISA kit (ab274402) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CA125 protein in serum, plasma, urine, cell culture supernatant, and milk. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CA125 with 0.02 U/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cancer antigen 125 (CA125, also known as Mucin 16) is a membrane associated cell surface protein containing approximately 22,096 amino acids. CA125 is the largest member of the Mucin family of proteins and is highly O-glycosylated. The hydrophilic nature of mucin proteins gives them the ability to form protective/lubricating gel-like barriers to protect against chemotherapy agents, foreign particles and infectious agents. CA125 is used as a biomarker for ovarian cancer detection, with about 90% of women with advanced ovarian cancer having elevated levels of CA125 present in their blood serum.
Product promise
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5 product images
Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (ab274402)
Human CA125 competitor curve comparison.
Standard Curve comparison between Human CA125 SimpleStep ELISA kit and traditional ELISA kit from leading competitor.
Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (ab274402)
Human CA125 standard curve comparison.
Standard Curve comparison between the original Human CA125 SimpleStep ELISA kit (ab195213) and current Human CA125 SimpleStep ELISA kit (ab274402).
Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (ab274402)
Example of human CA125 standard curve in Sample Diluent NS.
The CA125 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (ab274402)
Interpolated concentrations of native CA125 in Hela cell culture supernatant and milk samples.
The concentrations of CA125 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: Hela cell culture supernatant, 50%, milk, 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 3 U/mL in Hela cell culture supernatant, 4.5 U/mL in milk.
Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (ab274402)
Serum from eight individual healthy CA125 female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CA125 concentration was determined to be 0.2 U/mL, 6 donors CA125 were below detection level.
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