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AB218270

Human Calbindin ELISA Kit

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Human Calbindin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Calbindin with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CAB27, CALB1, Calbindin, Calbindin D28, D-28K

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Sandwich ELISA - Human Calbindin ELISA Kit (AB218270)
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Lab

Sandwich ELISA - Human Calbindin ELISA Kit (AB218270)

Human Calbindin standard curve comparison data.

Standard curve comparison between human Calbindin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 3-fold sensitivity.

Sandwich ELISA - Human Calbindin ELISA Kit (AB218270)
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Supplier Data

Sandwich ELISA - Human Calbindin ELISA Kit (AB218270)

Example of human calbindin standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Calbindin ELISA Kit (AB218270)
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Supplier Data

Sandwich ELISA - Human Calbindin ELISA Kit (AB218270)

Interpolated concentrations of native calbindin in human urine.

The concentrations of calbindin were measured in duplicate, interpolated from the calbindin standard curve and corrected for sample dilution. Undiluted sample was at 10%. The interpolated dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean calbindin concentration was determined to be 3.62 ng/mL in urine.

Sandwich ELISA - Human Calbindin ELISA Kit (AB218270)
  • sELISA

Supplier Data

Sandwich ELISA - Human Calbindin ELISA Kit (AB218270)

Interpolated concentrations of native calbindin in human brain tissue extract based on a 1,000 μg/mL extract load.

The concentrations of calbindin were measured in duplicate, interpolated from the calbindin standard curve, and corrected for sample dilution. The interpolated dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean calbindin concentration was determined to be 733 ng/mL of human brain tissue.

Sandwich ELISA - Human Calbindin ELISA Kit (AB218270)
  • sELISA

Supplier Data

Sandwich ELISA - Human Calbindin ELISA Kit (AB218270)

Example of human calbindin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.7 pg/mL

Range

9.4 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD66b antibody [EPR25354-2] (AB300122)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Calbindin ELISA Kit (ab218270) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Calbindin protein in tissue extracts and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Calbindin with 3.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Calbindin is a 260 amino acid protein encoded by the gene CALB1. Also, referred to as Calbindin D28, D-28K, and Vitamin D-dependent calcium-binding protein, CALB1 belongs to a family of calcium binding proteins which includes parvalbumin, calmodulin, calretinin S100G, and troponin C. CALB1 contains five EF-hand domains, and four calcium binding sites, although two sites have lost binding capacity for calcium. CALB1 functions in the absorption of calcium in the kidney and intestine, and in neural tissue CALB1 is involved in neural function by modulating calcium channel activity. Expressed in the distal tubule and in the proximal part of the collecting kidney ducts, CALB1 is thought to be a kidney injury marker.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.6" }, { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
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Recovery

[ { "sample": "Urine", "range": "114 - 117 %", "average": "= 115" }, { "sample": "Tissue Extracts", "range": "99 - 105 %", "average": "= 101" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calbindin also known as calbindin-D28k is a calcium-binding protein with a molecular mass of approximately 28 kDa. This target is mainly expressed in the neurons of the brain such as in the cerebellum and hippocampus as well as in the kidneys and intestine. Calbindin plays a mechanical role in binding and buffering intracellular calcium ions which is important for the regulation of calcium-dependent signaling pathways. Researchers often use techniques like Calbindin IHC (immunohistochemistry) and ELISA to study its distribution and presence within tissues.
Biological function summary

Calbindin contributes to cellular homeostasis by managing intracellular calcium levels. It does not appear to be part of a larger protein complex but operates independently. This protein helps to protect neurons from calcium overload potentially safeguarding against excitotoxicity which occurs when neurons die from excessive stimulation. Calbindin's function as a calcium buffer is of particular importance in maintaining neuron function and survival in areas with high neuronal activity.

Pathways

Calbindin has a significant role in the calcium signaling pathway and is also involved in the vitamin D pathway. The calcium signaling pathway is vital for many cellular processes like muscle contraction neurotransmitter release and gene expression. The vitamin D pathway regulates calcium and phosphate homeostasis in the body. Calbindin's relationship with proteins such as calmodulin and parvalbumin both involved in calcium regulation highlights its importance in maintaining cellular functions.

Abnormal Calbindin expression has been observed in conditions like epilepsy and Parkinson's disease. In epilepsy its decreased expression can impact the ability of neurons to regulate calcium properly leading to increased vulnerability to seizures. In Parkinson's disease altered Calbindin levels have been associated with the selective vulnerability of dopamine neurons. These diseases highlight the connection between Calbindin and proteins like alpha-synuclein known for its role in Parkinson's pathology emphasizing the need to understand its contribution to disease mechanisms.
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Product protocols

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Target data

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
See full target information CALB1
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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