Human Calcitonin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Calcitonin in Urine, Cell culture media, Serum, EDTA Plasma samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture media, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.7 - 300 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Calcitonin. Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed:35324283). Katacalcin. Katacalcin is a potent plasma calcium-lowering peptide.
Alternative names
CALC1, CALCA, Calcitonin
What's included?
Recommended products
Human Calcitonin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Calcitonin in Urine, Cell culture media, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture media, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.7 - 300 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Urine | n 8 | mean - | SD - | C.V. 4.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Urine | n 4 | mean - | SD - | C.V. 8.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 84 | Range 80 - 88 % |
Sample type EDTA Plasma | Average % = 85 | Range 83 - 90 % |
Sample type Urine | Average % = 103 | Range 99 - 107 % |
Sample type Cell culture media | Average % = 94 | Range 91 - 96 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Calcitonin ELISA kit (ab279411) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Calcitonin protein in human serum, plasma, urine, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Calcitonin with 1.6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Calcitonin is a 32- amino acid long peptide hormone that is proteolytically cleaved from a larger prepropeptide and is encoded by the CALCA gene. Calcitonin causes a rapid, short-lived reduction in the concentration of calcium and phosphate in blood by the incorporation of the ions into bone. Secretion of calcitonin by the C-cells of the thyroid gland is stimulated by an increase in calcium serum levels, as well as the gastrointestinal hormones, gastrin and pentagastrin. Calcitonin inhibits both osteoclast activity in bones and renal tubular cell reabsorption of calcium and phosphate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcitonin also known as thyrocalcitonin is a peptide hormone with a molecular mass of about 3.4 kDa. It consists of 32 amino acids and is primarily synthesized in parafollicular cells or C cells of the thyroid gland. This hormone regulates calcium and phosphate homeostasis in the body. It reduces blood calcium levels by inhibiting osteoclast activity in the bones and decreasing reabsorption in the kidneys leading to more calcium excretion. Calcitonin also interacts with its receptor a G protein-coupled receptor found in tissues like bone kidney and brain.
- Biological function summary
This hormone plays a significant role in maintaining calcium levels by balancing the actions of parathyroid hormone (PTH). Calcitonin works independently but interacts with the broader system managing bone remodeling through an intricate network. The presence of calcitonin in specialized receptor complexes allows it to exert its effects efficiently in target tissues making it an important player in calcium and phosphorus metabolism.
- Pathways
Calcitonin acts within the calcium signaling pathway and the bone resorption pathway. Through these pathways calcitonin works alongside other proteins such as parathyroid hormone (PTH) and calcitonin gene-related peptide (CGRP) maintaining skeletal integrity. It modulates the activity of osteoclasts ensuring that bone resorption activities are counterbalanced by bone formation and influences intracellular calcium concentration by binding to its receptor on target cells.
- Associated diseases and disorders
Calcitonin is linked to conditions such as osteoporosis and medullary thyroid carcinoma. Osteoporosis characterized by decreased bone density and increased fracture risk involves dysregulated calcium and phosphate balance. Proteins like estrogen and growth factors along with calcitonin influence bone health. Medullary thyroid carcinoma a type of thyroid cancer arising from C cells affects calcitonin levels with this hormone serving as a biomarker for diagnosing and monitoring treatment effectiveness. Calcitonin's connection with these diseases highlights its diagnostic and therapeutic potential.
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2 product images
Sandwich ELISA - Human Calcitonin ELISA Kit (ab279411)
Example of human Calcitonin standard curve in Sample Diluent NS.
The Calcitonin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Calcitonin ELISA Kit (ab279411)
Interpolated concentrations of native Calcitonin in human urine.
The concentrations of Calcitonin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 323 pg/mL in serum.
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