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AB234572

Human CAMK2B ELISA Kit

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(1 Publication)

Human CAMK2B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CAMK2B with a sensitivity of 1.28 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CAM2, CAMK2, CAMKB, CAMK2B, Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CaMK-II subunit beta

5 Images
Sandwich ELISA - Human CAMK2B ELISA Kit (AB234572)
  • sELISA

Supplier Data

Sandwich ELISA - Human CAMK2B ELISA Kit (AB234572)

Interpolated concentrations of native CAMK2B in human brain tissue extract, based on a 100 μg/mL extract load.

The concentrations of CAMK2B were measured in duplicate and interpolated from the CAMK2B standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CAMK2B concentration was determined to be 37 ng/mL in human brain tissue extract.

Sandwich ELISA - Human CAMK2B ELISA Kit (AB234572)
  • sELISA

Supplier Data

Sandwich ELISA - Human CAMK2B ELISA Kit (AB234572)

Interpolated concentrations of native CAMK2B in human SH-SY5Y cell extract, HEK293T cell extract, MCF-7 cell extract, A431 cell extract and A20 cell extract, based on a 500 μg/mL extract load.

The concentrations of CAMK2B were measured in duplicate and interpolated from the CAMK2B standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CAMK2B concentration was determined to be 200 ng/mL in SH-SY5Y cell extract, 64 ng/mL in HEK293T cell extract, 52 ng/mL in MCF-7 cell extract, 76 ng/mL in A431 cell extract and 13 ng/mL in A20 cell extract.

Sandwich ELISA - Human CAMK2B ELISA Kit (AB234572)
  • sELISA

Supplier Data

Sandwich ELISA - Human CAMK2B ELISA Kit (AB234572)

Representative image of Human CAMK2B ELISA Kit (ab234572)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human CAMK2B ELISA Kit Capture Antibody

- 10X Human CAMK2B Detector Antibody

- 10X Human CAMK2B ELISA Kit Lyophilised Purified Protein (2 vials)

- Human CAMK2B ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human CAMK2B ELISA Kit (AB234572)
  • sELISA

Supplier Data

Sandwich ELISA - Human CAMK2B ELISA Kit (AB234572)

Interpolated concentrations of native CAMK2B in mouse RAW 264.7 cell extract and rat PC-12 cell extract based on a 500 μg/mL extract load.

The concentrations of CAMK2B were measured in duplicate and interpolated from the CAMK2B standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CAMK2B concentration was determined to be 73 ng/mL in mouse RAW 264.7 cell extract and 195 ng/mL in rat PC-12 cell extract.

Sandwich ELISA - Human CAMK2B ELISA Kit (AB234572)
  • sELISA

Supplier Data

Sandwich ELISA - Human CAMK2B ELISA Kit (AB234572)

Example of human CAMK2B standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.28 ng/mL

Range

3.13 - 200 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CAMK2B ELISA Kit (ab234572) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CAMK2B protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CAMK2B with 1.28 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "98 - 99 %", "average": "= 98" }, { "sample": "Tissue Extracts", "range": "102 - 109 %", "average": "= 106" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234572-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234572-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234572-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB234572-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234572-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234572-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234572-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CAMK2B Lyophilized recombinant Protein", "number":"AB234572-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB234572-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CAMK2B Capture Antibody", "number":"AB234572-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB234572-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CAMK2B Detector Antibody", "number":"AB234572-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234572-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CaMKII beta also called CaM-K 2B is a calcium/calmodulin-dependent protein kinase involved in many cellular processes. It is known for its molecular weight of approximately 73 kDa. Expressed widely in tissues CaMKII beta is particularly important in brain and heart tissues. CaMKII family which includes CaMK plays a role in catalyzing the phosphorylation of serine and threonine residues in proteins influencing varied cellular activities.
Biological function summary

Without referring directly to CaMKII beta this protein contributes to synaptic plasticity which influences learning and memory in neural cells. It functions as part of a multimeric complex forming a holoenzyme with other CaMKII isoforms like CaMKII alpha gamma and delta. The structural dynamics of its autophosphorylation regulate its prolonged activity independent of calcium and calmodulin affecting downstream signaling.

Pathways

Without directly mentioning CaMKII beta it is essential in the Ca2+/calmodulin signaling pathway critical for regulating neuronal activity. Additionally this kinase participates in the cardiac conduction system influencing the heartbeat. Related proteins like calcineurin and protein kinase A interact in these signaling pathways amplifying the role of CaMKII beta in translating calcium bursts into longer-term cellular responses.

Without direct mention CaMKII beta's involvement is evident in conditions such as Alzheimer's disease and arrhythmias. In neurodegenerative diseases its deregulation affects the balance of phosphorylation involving proteins like tau which contribute to disease progression. In cardiac disorders its role in arrhythmias interacts with ion channels affecting heart rhythm stability. Understanding these interactions helps in therapeutic approaches targeting CaMKII beta-related pathways.
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Product protocols

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Target data

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle (PubMed : 16690701). In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed : 29100089). Participates in the modulation of skeletal muscle function in response to exercise (PubMed : 16690701). In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (By similarity). Phosphorylates reticulophagy regulator RETREG1 at 'Ser-151' under endoplasmic reticulum stress conditions which enhances RETREG1 oligomerization and its membrane scission and reticulophagy activity (PubMed : 31930741).
See full target information CAMK2B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proteomics 19:e1800164 PubMed30536821

2018

Quantitative Proteomics by SWATH-MS Suggest an Association Between Circulating Exosomes and Maternal Metabolic Changes in Gestational Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Nanthini Jayabalan,Andrew Lai,Soumyalekshmi Nair,Dominic Guanzon,Katherin Scholz-Romero,Carlos Palma,Harold David McIntyre,Martha Lappas,Carlos Salomon
View all publications
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websiteProtocolBooklet
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