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AB314827

Human Carbonic Anhydrase 1/CA1 ELISA Kit

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Human Carbonic Anhydrase 1/CA1 ELISA Kit is a Sandwich ELISA for the measurement of Human Carbonic Anhydrase 1/CA1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Carbonic anhydrase 1, Carbonate dehydratase I, Carbonic anhydrase B, Carbonic anhydrase I, Cyanamide hydratase CA1, CAB, CA-I, CA1

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Sandwich ELISA - Human Carbonic Anhydrase 1/CA1 ELISA Kit (AB314827)
  • sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 1/CA1 ELISA Kit (AB314827)

Example of standard curve using ab314827. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.09 pg/mL

Range

0.09 - 20 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB267475

Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (AB267475)

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Biochemicals

AB120573

L-DOPA, Dopamine (ab120565) precursor

Chemical Structure - L-DOPA, Dopamine (ab120565) precursor (AB120573)

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Proteins & Peptides

AB208320

Recombinant human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus) (AB208320)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Carbonic Anhydrase 1/CA1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Carbonic Anhydrase 1/CA1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Carbonic Anhydrase 1/CA1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "82 - 94 %", "average": "= 88.21" }, { "sample": "Plasma", "range": "81 - 94 %", "average": "= 88.06" }, { "sample": "Cell culture media", "range": "80 - 113 %", "average": "= 94.13" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314827-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human Carbonic Anhydrase 1/CA1 Protein Standard", "number":"AB314827-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Carbonic Anhydrase 1/CA1 Detection Antibody", "number":"AB314827-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314827-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314827-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314827-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB314827-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314827-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Carbonic Anhydrase 1/CA1 Antibody-coated ELISA Plate", "number":"AB314827-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB314827-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Catalyzes the reversible hydration of carbon dioxide (PubMed : 10550681, PubMed : 16506782, PubMed : 16686544, PubMed : 16807956, PubMed : 17127057, PubMed : 17314045, PubMed : 17407288, PubMed : 18618712, PubMed : 19186056, PubMed : 19206230). Can hydrate cyanamide to urea (PubMed : 10550681).
See full target information Carbonic anhydrase 1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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