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AB283996

Human Carbonic Anhydrase 14/CA14 ELISA Kit

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Human Carbonic Anhydrase 14/CA14 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Carbonic Anhydrase 14/CA14 in Human in Cell/Tissue Extracts samples.

View Alternative Names

UNQ690/PRO1335, CA14, Carbonic anhydrase 14, Carbonate dehydratase XIV, Carbonic anhydrase XIV, CA-XIV

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Sandwich ELISA - Human Carbonic Anhydrase 14/CA14 ELISA Kit (AB283996)
  • sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 14/CA14 ELISA Kit (AB283996)

Example of Human Carbonic Anhydrase 14/CA14 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.15 ng/mL

Range

0.313 - 20 ng/mL

Assay time

4h

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Carbonic Anhydrase 14 / CA14 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for detection of Carbonic Anhydrase (CA14) in human cell lysate samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CA14 in approximately 4 hours. A polyclonal antibody specific for human CA14 has been pre-coated onto a 96-well microplate with removable strips. CA14 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CA14, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283996-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283996-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283996-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Chromogen Substrate", "number":"AB283996-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Carbonic Anhydrase 14/CA14 Antibody", "number":"AB283996-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB283996-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Carbonic Anhydrase 14/CA14 Microplate", "number":"AB283996-CMP05", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283996-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283996-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283996-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Carbonic Anhydrase 14/CA14 Standard", "number":"AB283996-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Reversible hydration of carbon dioxide.
See full target information CA14
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websiteProtocolBooklet
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