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AB222881

Human Carbonic Anhydrase 2 ELISA Kit

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(3 Publications)

Human Carbonic Anhydrase 2 ELISA Kit is a Sandwich ELISA for the measurement of Human Carbonic Anhydrase 2 in Human in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.

View Alternative Names

Carbonic anhydrase 2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II, Cyanamide hydratase CA2, CAC, CA-II, CA2

1 Images
Sandwich ELISA - Human Carbonic Anhydrase 2 ELISA Kit (AB222881)
  • sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 2 ELISA Kit (AB222881)

Example of human CA2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Tissue, Cell culture supernatant, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 21 pg/mL

Range

0.031 - 2 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human CA2 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222881) is designed for detection of human CA2 in plasma, serum, and cell culture samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CA2 in approximately 4 hours. A polyclonal antibody specific for human CA2 has been pre-coated onto a 96-well microplate with removable strips. CA2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CA2, which is recognized by a Streptavidin-Peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Carbonic anhydrase 2 (CA2, CAC), also known as carbonate dehydratase II and carbonic anhydrase II, belongs to the alpha-carbonic anhydrase family. It contains 259 amino acid residues and has a molecular mass of 29 kDa. CA2 catalyzes reversible hydration of carbon dioxide and is expressed at high levels in osteoclasts during bone resorption. CA2 supports the transport of bicarbonate ions, sodium ions, and water from the blood to the cerebrospinal fluid.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab222881-1x96Tests", "assetComponentDetails": [ { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB222881-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human CA2 Standard", "number":"AB222881-CMP06", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB222881-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB222881-CMP04", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB222881-CMP07", "productcode":"" }, { "size":"1 x 3 Unit", "name":"Sealing Tapes", "number":"AB222881-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB222881-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB222881-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti- Human CA2 coated Microplate (12 x 8 wells)", "number":"AB222881-CMP01", "productcode":"" }, { "size":"1 x 75 µL", "name":"80X Biotinylated Human CA2", "number":"AB222881-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Catalyzes the reversible hydration of carbon dioxide (PubMed : 11327835, PubMed : 11802772, PubMed : 11831900, PubMed : 12056894, PubMed : 12171926, PubMed : 1336460, PubMed : 14736236, PubMed : 15300855, PubMed : 15453828, PubMed : 15667203, PubMed : 15865431, PubMed : 16106378, PubMed : 16214338, PubMed : 16290146, PubMed : 16686544, PubMed : 16759856, PubMed : 16807956, PubMed : 17127057, PubMed : 17251017, PubMed : 17314045, PubMed : 17330962, PubMed : 17346964, PubMed : 17540563, PubMed : 17588751, PubMed : 17705204, PubMed : 18024029, PubMed : 18162396, PubMed : 18266323, PubMed : 18374572, PubMed : 18481843, PubMed : 18618712, PubMed : 18640037, PubMed : 18942852, PubMed : 1909891, PubMed : 1910042, PubMed : 19170619, PubMed : 19186056, PubMed : 19206230, PubMed : 19520834, PubMed : 19778001, PubMed : 7761440, PubMed : 7901850, PubMed : 8218160, PubMed : 8262987, PubMed : 8399159, PubMed : 8451242, PubMed : 8485129, PubMed : 8639494, PubMed : 9265618, PubMed : 9398308). Can also hydrate cyanamide to urea (PubMed : 10550681, PubMed : 11015219). Stimulates the chloride-bicarbonate exchange activity of SLC26A6 (PubMed : 15990874). Essential for bone resorption and osteoclast differentiation (PubMed : 15300855). Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption.
See full target information CA2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 14:8686-8694 PubMed36628213

2022

Diagnostic role of urinary CA-2 in urinary stones and its prediction of complications.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanjing Leng,Haibin Zhou,Wenjuan Wang,Jiangling Fu

Journal of clinical medicine 10: PubMed33917571

2021

Lymphaticovenous Anastomosis Supermicrosurgery Decreases Oxidative Stress and Increases Antioxidant Capacity in the Serum of Lymphedema Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Johnson Chia-Shen Yang,Lien-Hung Huang,Shao-Chun Wu,Pao-Jen Kuo,Yi-Chan Wu,Chia-Jung Wu,Chia-Wei Lin,Pei-Yu Tsai,Ching-Hua Hsieh

Cell 182:1044-1061.e18 PubMed32795414

2020

Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ayuko Hoshino,Han Sang Kim,Linda Bojmar,Kofi Ennu Gyan,Michele Cioffi,Jonathan Hernandez,Constantinos P Zambirinis,Gonçalo Rodrigues,Henrik Molina,Søren Heissel,Milica Tesic Mark,Loïc Steiner,Alberto Benito-Martin,Serena Lucotti,Angela Di Giannatale,Katharine Offer,Miho Nakajima,Caitlin Williams,Laura Nogués,Fanny A Pelissier Vatter,Ayako Hashimoto,Alexander E Davies,Daniela Freitas,Candia M Kenific,Yonathan Ararso,Weston Buehring,Pernille Lauritzen,Yusuke Ogitani,Kei Sugiura,Naoko Takahashi,Maša Alečković,Kayleen A Bailey,Joshua S Jolissant,Huajuan Wang,Ashton Harris,L Miles Schaeffer,Guillermo García-Santos,Zoe Posner,Vinod P Balachandran,Yasmin Khakoo,G Praveen Raju,Avigdor Scherz,Irit Sagi,Ruth Scherz-Shouval,Yosef Yarden,Moshe Oren,Mahathi Malladi,Mary Petriccione,Kevin C De Braganca,Maria Donzelli,Cheryl Fischer,Stephanie Vitolano,Geraldine P Wright,Lee Ganshaw,Mariel Marrano,Amina Ahmed,Joe DeStefano,Enrico Danzer,Michael H A Roehrl,Norman J Lacayo,Theresa C Vincent,Martin R Weiser,Mary S Brady,Paul A Meyers,Leonard H Wexler,Srikanth R Ambati,Alexander J Chou,Emily K Slotkin,Shakeel Modak,Stephen S Roberts,Ellen M Basu,Daniel Diolaiti,Benjamin A Krantz,Fatima Cardoso,Amber L Simpson,Michael Berger,Charles M Rudin,Diane M Simeone,Maneesh Jain,Cyrus M Ghajar,Surinder K Batra,Ben Z Stanger,Jack Bui,Kristy A Brown,Vinagolu K Rajasekhar,John H Healey,Maria de Sousa,Kim Kramer,Sujit Sheth,Jeanine Baisch,Virginia Pascual,Todd E Heaton,Michael P La Quaglia,David J Pisapia,Robert Schwartz,Haiying Zhang,Yuan Liu,Arti Shukla,Laurence Blavier,Yves A DeClerck,Mark LaBarge,Mina J Bissell,Thomas C Caffrey,Paul M Grandgenett,Michael A Hollingsworth,Jacqueline Bromberg,Bruno Costa-Silva,Hector Peinado,Yibin Kang,Benjamin A Garcia,Eileen M O'Reilly,David Kelsen,Tanya M Trippett,David R Jones,Irina R Matei,William R Jarnagin,David Lyden
View all publications
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