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AB283977

Human Carbonic Anhydrase 3/CA3 ELISA Kit

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Human Carbonic Anhydrase 3/CA3 ELISA Kit is a Sandwich ELISA for the measurement of Human Carbonic Anhydrase 3/CA3 in Human in Cell/Tissue Extracts, Biofluids, Cell Culture Media samples.

View Alternative Names

Carbonic anhydrase 3, Carbonate dehydratase III, Carbonic anhydrase III, CA-III, CA3

1 Images
Sandwich ELISA - Human Carbonic Anhydrase 3/CA3 ELISA Kit (AB283977)
  • sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 3/CA3 ELISA Kit (AB283977)

Example of Human Carbonic Anhydrase 3/CA3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Urine, Plasma, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.17 ng/mL

Range

0.25 - 16 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Carbonic Anhydrase 3/CA3 ELISA Kit (ab283977) is designed for detection of CA3 in human plasma, serum, urine, cell culture, and cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CA3 in approximately 4 hours.

A polyclonal antibody specific for human CA3 has been pre-coated onto a 96-well microplate with removable strips. CA3 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CA3, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283977-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283977-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB283977-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB283977-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Carbonic Anhydrase 3/CA3 Antibody", "number":"AB283977-CMP07", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283977-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Carbonic Anhydrase 3/CA3 Standard", "number":"AB283977-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Carbonic Anhydrase 3/CA3 Microplate", "number":"AB283977-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283977-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283977-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB283977-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Reversible hydration of carbon dioxide.
See full target information CA3
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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