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AB275899

Human Carbonic Anhydrase 6 (CA6) ELISA Kit

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Human Carbonic Anhydrase 6 (CA6) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Carbonic Anhydrase 6 (CA6) with a sensitivity of 2.12 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Carbonic anhydrase 6, Carbonate dehydratase VI, Carbonic anhydrase VI, Salivary carbonic anhydrase, Secreted carbonic anhydrase, CA-VI, CA6

4 Images
Sandwich ELISA - Human Carbonic Anhydrase 6 (CA6) ELISA Kit (AB275899)
  • sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 6 (CA6) ELISA Kit (AB275899)

Interpolated concentrations of native Carbonic Anhydrase 6 in human kidney sample based on a 500 μg/mL extract load.

The concentrations of Carbonic Anhydrase 6 were measured in duplicate and interpolated from the Carbonic Anhydrase 6 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Carbonic Anhydrase 6 concentration was determined to be 32.4 pg/mL in kidney sample.

Sandwich ELISA - Human Carbonic Anhydrase 6 (CA6) ELISA Kit (AB275899)
  • sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 6 (CA6) ELISA Kit (AB275899)

Interpolated concentrations of native Carbonic Anhydrase 6 in human serum, plasma, milk, and saliva samples.

The concentrations of Carbonic Anhydrase 6 were measured in duplicates, interpolated from the Carbonic Anhydrase 6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 100, milk 1 : 10,000, and saliva 1 : 25,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Carbonic Anhydrase 6 concentration was determined to be 87.8 ng/mL in serum, 97.4 ng/mL in plasma (citrate), 6,159 ng/mL in milk, and 10,255 ng/mL in saliva.

Sandwich ELISA - Human Carbonic Anhydrase 6 (CA6) ELISA Kit (AB275899)
  • sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 6 (CA6) ELISA Kit (AB275899)

Example of human Carbonic Anhydrase 6 standard curve in Sample Diluent NS.

The Carbonic Anhydrase 6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Carbonic Anhydrase 6 (CA6) ELISA Kit (AB275899)
  • sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 6 (CA6) ELISA Kit (AB275899)

Example of human Carbonic Anhydrase 6 standard curve in 1X Cell Extraction Buffer PTR.

The Carbonic Anhydrase 6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Citrate plasma, Milk, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.12 pg/mL

Range

15.63 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human Carbonic anhydrase 6 ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Carbonic anhydrase 6 protein in human serum, plasma, milk, saliva, and cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Carbonic anhydrase 6 with 2.12 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Carbonic anhydrase 6 (CA6), encoded by CA6 gene, is a zinc metalloenzyme. More specifically, an extracellular secretory isoform of the CA family that catalyzes the reversible hydration-dehydration of CO2 and HCO3-1: CO2 + H2O <=> H+ + HCO3- and found in all tissue and cell types. CA6 main presence is found in saliva, though its function in saliva is unknown.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]

Recovery

[ { "sample": "Milk", "range": "102 - 102 %", "average": "= 102" }, { "sample": "Serum", "range": "100 - 109 %", "average": "= 104" }, { "sample": "Saliva", "range": "93 - 96 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "106 - 112 %", "average": "= 109" }, { "sample": "Tissue Extracts", "range": "87 - 100 %", "average": "= 95" } ]

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

Target data

Reversible hydration of carbon dioxide. Its role in saliva is unknown.
See full target information CA6
websiteProtocolBooklet
en

Product promise

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