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AB289905

Human Carbonic Anhydrase 9/CA9 ELISA Kit

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Human Carbonic Anhydrase 9/CA9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Carbonic Anhydrase 9/CA9 with a sensitivity of 3.141 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250

3 Images
Sandwich ELISA - Human Carbonic Anhydrase 9/CA9 ELISA Kit (AB289905)
  • sELISA

Unknown

Sandwich ELISA - Human Carbonic Anhydrase 9/CA9 ELISA Kit (AB289905)

Interpolated concentrations of human Carbonic Anhydrase 9/CA9 in HT-29 Supernatant.

Interpolated concentration of native Carbonic Anhydrase 9/CA9 was measured in duplicate at different sample concentrations. Undiluted samples are 20% HT-29 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Carbonic Anhydrase 9/CA9 ELISA Kit (AB289905)
  • sELISA

Unknown

Sandwich ELISA - Human Carbonic Anhydrase 9/CA9 ELISA Kit (AB289905)

Human Carbonic Anhydrase 9/CA9 competitor standard curve comparison.

Standard curve comparison between Human Carbonic Anhydrase 9/CA9 SimpleStep ELISA kit (ab289905) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows comparable sensitivity.

Sandwich ELISA - Human Carbonic Anhydrase 9/CA9 ELISA Kit (AB289905)
  • sELISA

Unknown

Sandwich ELISA - Human Carbonic Anhydrase 9/CA9 ELISA Kit (AB289905)

Example of human Carbonic Anhydrase 9/CA9 standard curve in Sample Diluent NS.

Example of human Carbonic Anhydrase 9/CA9 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

>= 3.141 pg/mL

Range

19.531 - 1250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Carbonic Anhydrase 9/CA9 ELISA Kit (ab289905) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Carbonic Anhydrase 9/CA9 in cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Carbonic Anhydrase 9/CA9 with 3.141 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.7" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 120 %", "average": "= 109" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
See full target information CA9
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websiteProtocolBooklet
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Product promise

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