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AB314601

Human Carboxypeptidase A ELISA Kit

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Human Carboxypeptidase A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Carboxypeptidase A with a sensitivity of 5.743 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CPA, CPA1, Carboxypeptidase A1

2 Images
Sandwich ELISA - Human Carboxypeptidase A ELISA Kit (AB314601)
  • sELISA

Supplier Data

Sandwich ELISA - Human Carboxypeptidase A ELISA Kit (AB314601)

Interpolated concentration of native Carboxypeptidase A was measured in duplicate at different sample concentrations. Undiluted sample is 150 ng/mL Human Pancreas Tissue Extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent PTR

Sandwich ELISA - Human Carboxypeptidase A ELISA Kit (AB314601)
  • sELISA

Supplier Data

Sandwich ELISA - Human Carboxypeptidase A ELISA Kit (AB314601)

Example of human Carboxypeptidase A standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.743 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Carboxypeptidase A SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Carboxypeptidase A protein in Tissue extracts . Quantitate Human Carboxypeptidase A with 5.743 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Cell Lysate", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Inter", "sample": "Cell Lysate", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.01" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "104 - 114 %", "average": "= 109" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314601-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB314601-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB314601-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB314601-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Carboxypeptidase A Capture Antibody", "number":"AB314601-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB314601-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB314601-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Carboxypeptidase A Lyophilized Recombinant Protein", "number":"AB314601-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Carboxypeptidase A Detector Antibody", "number":"AB314601-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB314601-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB314601-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB314601-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (PubMed : 20385563, PubMed : 8806703). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (By similarity).
See full target information CPA1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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