AB99992

Human Carcino Embryonic Antigen ELISA Kit (CEA)

Name: Human Carcino Embryonic Antigen ELISA Kit (CEA) (ab99992) | Abcam
Brand: Abcam Limited
SKU: AB99992
Price: 780 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(12 Publications)

Human Carcino Embryonic Antigen ELISA Kit (CEA) is a Sandwich ELISA for the measurement of Human Carcino Embryonic Antigen (CEA) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5

3 Images

Sandwich ELISA - Human Carcino Embryonic Antigen ELISA Kit (CEA) (AB99992)

sELISA

Supplier Data

Sandwich ELISA - Human Carcino Embryonic Antigen ELISA Kit (CEA) (AB99992)

Representative Standard Curve using ab99992.

ELISA

Lab

ELISA - Human Carcino Embryonic Antigen ELISA Kit (CEA) (AB99992)

CEA measured in biological fluids showing quantity (ng) per mL of tested sample

sELISA

Supplier Data

Sandwich ELISA - Human Carcino Embryonic Antigen ELISA Kit (CEA) (AB99992)

Representative Standard Curve using ab99992.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.2 ng/mL

Range

0.343 - 250 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Carcinoembryonic Antigen (CEA) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEA in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human Carcinoembryonic Antigen ELISA Kit (CD66e) (ab183365) from our SimpleStep ELISA^® range.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 136 %", "average": "= 116.7" }, { "sample": "Serum", "range": "135 - 156 %", "average": "= 145.6" }, { "sample": "Plasma", "range": "82 - 124 %", "average": "= 103.8" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99992-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99992-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99992-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human CEA Standard (lyophilized)", "number":"AB99992-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CEA Microplate (12 x 8 well strips)", "number":"AB99992-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99992-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99992-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human CEA", "number":"AB99992-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"1,000X HRP-Streptavidin Concentrate", "number":"AB99992-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99992-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed : 10864933, PubMed : 10910050, PubMed : 2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed : 2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed : 10910050).. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.

See full target information CEACAM5

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

International journal of pharmaceutics: X 9:100311 PubMed39811247

2025

Phloretin-nanospanlastics for targeting the Akt/PI3K signaling pathways in dimethylhydrazine-induced colon cancer in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ebtsam A Abdel-Wahab,Zahraa Haleem Al-Qaim,Ahmed T H Faris Al-Karkhi,Aysam M Fayed,Ahmed M Eldmrdash,Mohammed Abdalla Hussein,Amal Abdel-Aziz,Azza M Metwaly,Heba G Abdelzaher,M A Abdelzaher,Diana A ALsherif

Journal of infection and public health 15:1315-1320 PubMed36279687

2022

Differential expression of carcinoembryonic antigen-related cell adhesion molecule-5 (CEACAM5) and dipeptidyl peptidase-4 (DPP4) with detection of Middle East respiratory syndrome-coronavirus in peripheral blood.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulkarim Alhetheel,Ahmed Albarrag,Zahid Shakoor,Ali Somily,Mazin Barry,Hifa Altalhi,Muhammed Bakhrebah,Majed Nassar,Mohamed Alfageeh,Ayed Assiri,Sarah Alfaraj,Ziad A Memish

Scientific reports 12:15315 PubMed36097151

2022

Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors.

Applications

Unspecified application

Species

Unspecified reactive species

Sowmya Joshi,Shashidhar Kallappa,Pranjal Kumar,Sudhanshu Shukla,Ruma Ghosh

Computational intelligence and neuroscience 2022:4540176 PubMed36052030

2022

Detection Rate and Prognosis of Lymph Nodes in Gastric Cancer Using Nano Carbon Combined with Anatomical Sorting.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ma,Chongren Ren,Gang Du,Jinxi Wang,Zhenguo Han,Huhu Li,Huiyu Li,Liang Xue

Turkish journal of medical sciences 51:3061-3066 PubMed34519193

2021

Calcitonin levels in washout samples vs. cytology in the detection of malignant lymph node metastasis in recurrent medullary thyroid cancer

Applications

Unspecified application

Species

Unspecified reactive species

Asena Gökçay Canpolat,Mustafa Şahin,Koray Ceyhan,Serpil Sak,Özgür Demir,Rıfat Emral,Murat Faik Erdoğan,Sevim Güllü,Demet Çorapçıoğlu

Clinical and translational gastroenterology 12:e00403 PubMed34644276

2021

Liquid Biopsy in Gastric Cancer: Analysis of Somatic Cancer Tissue Mutations in Plasma Cell-Free DNA for Predicting Disease State and Patient Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Greta Varkalaite,Michael Forster,Andre Franke,Juozas Kupcinskas,Jurgita Skieceviciene

Micromachines 12: PubMed34683189

2021

4-Nitrophenol-Loaded Magnetic Mesoporous Silica Hybrid Materials for Spectrometric Aptasensing of Carcinoembryonic Antigen.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Zhang,Dianping Tang

Computational and mathematical methods in medicine 2021:9987067 PubMed34257703

2021

Construction and Validation of a Lung Cancer Diagnostic Model Based on 6-Gene Methylation Frequency in Blood, Clinical Features, and Serum Tumor Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Kang,Dandan Wang,Xiuzhi Zhang,Lingxiao Wang,Fengxiang Wang,Jie Chen

Scientific reports 8:10002 PubMed29968766

2018

Validation of Assaying Carcinoembryonic Antigen in Human Serum by Using Immunomagnetic Reduction.

Applications

Unspecified application

Species

Unspecified reactive species

Po-Li Wei,Long-Teng Lee,Li-Ming Tseng,Kai-Wen Huang

Oncology letters 14:6409-6416 PubMed29422956

2017

MDM2 binding protein as a predictor of metastasis and a novel prognostic biomarker in patients with gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Zhong Chen,Jiejie Jin,Ziwen Long,Xiaowen Liu,Hong Cai,Ye Zhou,Hua Huang,Yanong Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Carcino Embryonic Antigen ELISA Kit (CEA)

Sandwich ELISA - Human Carcino Embryonic Antigen ELISA Kit (CEA) (AB99992)

ELISA - Human Carcino Embryonic Antigen ELISA Kit (CEA) (AB99992)

Sandwich ELISA - Human Carcino Embryonic Antigen ELISA Kit (CEA) (AB99992)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (12)

Product promise