Human Cardiac Troponin I ELISA Kit
- Recombinant
- SimpleStep
- What is this?
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(2 Reviews)
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(14 Publications)
Human Cardiac Troponin I ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cardiac Troponin I with a sensitivity of 4.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
View Alternative Names
TNNC1, TNNI3, Cardiac troponin I
- ELISA
Supplier Data
ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Interpolated concentrations of native Cardiac Troponin 1 in human heart tissue extract based on a 10 μg/mL extract load.
The concentrations of Cardiac Troponin 1 were measured in duplicate and interpolated from the Cardiac Troponin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin 1 concentration was determined to be 1,793 pg/mL in human heart tissue extract.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Example of human Cardiac Troponin 1 standard curve in 1X Cell Extraction Buffer PTR.
The Cardiac Troponin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Recombinant Cardiac Troponin 1 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent 50BP for serum and plasma samples and Sample Diluent NS for cell culture media samples.
Neat pooled serum and plasma (EDTA, Heparin, Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Example of human Cardiac Troponin 1 standard curve in Sample Diluent 50BP.
The Cardiac Troponin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Example of human Cardiac Troponin 1 standard curve in Sample Diluent NS.
The Cardiac Troponin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Sensitivity.
The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Comparison of Cardiac Troponin 1 in various human tissue extracts.
Human heart was analyzed based on a 10 μg/mL extract load while human skeletal muscle and human brain extracts were analyzed based on a 200 μg/mL extract load. The concentrations of Cardiac Troponin 1 were measured in duplicate and interpolated from the Cardiac Troponin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin 1 concentration was determined to be 182.4 ng/mg in human heart tissue extract and undetectable in human skeletal muscle and brain tissue extracts.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Example of human Cardiac Troponin 1 standard curve in 1X Cell Extraction Buffer PTR. The Cardiac Troponin 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Linearity of dilution.
Neat serum from ten individual healthy human female donors was measured in duplicate. All values were below the detectable range of the assay.
Native Cardiac Troponin 1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Human Cardiac Troponin 1 Standard Curve Comparison between current and original ELISA kit.
Standard Curve comparison between current human Cardiac Troponin 1 SimpleStep ELISA kit and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Example of human Cardiac Troponin 1 standard curve in Sample Diluent NS. The Cardiac Troponin 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)
Example of human Cardiac Troponin 1 standard curve in Sample Diluent 50BP. The Cardiac Troponin 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human Cardiac Troponin I ELISA Kit ab200016 is a rapid single-wash 90-min Sandwich ELISA to measure Human Cardiac Troponin I in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 4.4 pg/mL.
How the assay works
Human Cardiac Troponin I SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Cardiac Troponin I ELISA Kit ab200016 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
The regulatory troponin complex regulates skeletal and cardiac muscle contraction. This complex, together with tropomyosin, is located on the actin filament and it is composed of three protein subunits: troponin T (the tropomyosin-binding subunit), troponin I (the inhibitory subunit, which inhibits the ATPase activity of acto-myosin), and troponin C (the Ca2+-binding subunit). Troponins T and I have unique cardiac isoforms, whereas cardiac and skeletal muscle share troponin C. Specifically, three human troponin I isoforms have been described: one is expressed in cardiac muscle (Cardiac Troponin I) and the other two are found in slow-twitch and fast-twitch skeletal muscle fibers (slow sTnI and fast sTnI, respectively). The sequence homology between Cardiac Troponin I and slow sTnI is approximately 40% and somewhat less for fast sTnI. Cardiac Troponin I is 209 amino acid long with a molecular weight of approximately 24 kDa. Mouse and rat Cardiac Troponin I proteins both show 93% amino acid identity to human Cardiac Troponin I. The presence of human Cardiac Troponin I in serum (together with chest pain and electrocardiographic changes) is now considered as one highly specific biochemical marker of myocardial injury, risk stratification of acute coronary syndrome and myocardial infarction. Mutations of Cardiac Troponin I are associated with hereditary cardiomyopathy. Specifically, defects in Cardiac Troponin I are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intra-familial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in Cardiac Troponin I also cause cardiomyopathy familial restrictive type 1 (RCM1). RCM1 is a heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. Furthermore, cardiomyopathy dilated type 2A (CMD2A) and cardiomyopathy dilated type 1FF (CMD1FF), disorders characterized by ventricular dilation and impaired systolic function resulting in congestive heart failure and arrhythmia, are caused by defects in Cardiac Troponin I.
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Publications (14)
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Signal transduction and targeted therapy 10:157 PubMed40360476
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iScience 27:109139 PubMed38384853
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ACS sensors 8:3574-3584 PubMed37705448
2023
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Journal of molecular recognition : JMR 36:e2995 PubMed36116102
2022
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Nature biomedical engineering 6:351-371 PubMed35478225
2022
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Experimental biology and medicine (Maywood, N.J.) 247:1205-1213 PubMed35466734
2022
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Analytical and bioanalytical chemistry 414:5319-5327 PubMed34595559
2021
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Journal of cardiovascular translational research 15:381-390 PubMed34417673
2021
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Biomolecules 10: PubMed32784904
2020
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Nanomaterials (Basel, Switzerland) 10: PubMed32708486
2020
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