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AB326433

Human Cardiac Troponin I ELISA Kit, Chemiluminescent

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Human Cardiac Troponin I ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Cardiac Troponin I with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 5.49 - 12000 pg/ml with a sensitivity of 0.78pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

6 Images
Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)
  • sELISA

Lab

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)
  • sELISA

Lab

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

Standard curve comparison between the Chemiluminescent Human Cardiac Troponin 1 SimpleStep ELISA and colorimetric Human Cardiac Troponin 1 SimpleStep ELISA (ab200016) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)
  • sELISA

Lab

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

Dynamic range comparison between the Chemiluminescent Human Cardiac Troponin 1 SimpleStep ELISA and colorimetric Human Cardiac Troponin 1 SimpleStep ELISA (ab200016). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

Sensitivity.

The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

Linearity of dilution.

Neat serum from ten individual healthy human female donors was measured in duplicate. All values were below the detectable range of the assay.

Native Cardiac Troponin 1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant Cardiac Troponin 1 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent 50BP for serum and plasma samples and Sample Diluent NS for cell culture media samples.

Neat pooled serum and plasma (EDTA Heparin Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.78 pg/mL

Range

5.49 - 12000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Cardiac Troponin I ELISA Kit, Chemiluminescent (ab326433) is a rapid single-wash sandwich 55-min ELISA to measure Human Cardiac Troponin I in Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 0.78 pg/mL.

How the assay works

Human Cardiac Troponin I ELISA Kit, Chemiluminescent (ab326433) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Cardiac Troponin I (ab326433) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TNNI3 antibody [EPR20307-239] - BSA and Aside free (Capture) ab244634
- Anti-TNNI3 antibody [EPR20307-55] - BSA and Aside free (Detector) ab244896
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Recovery

[ { "sample": "Serum", "range": "93 - 101 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "90 - 97 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "99 - 117 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "87 - 100 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "97 - 108 %", "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNI3
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com