AB223860

Human Cardiac Troponin T ELISA Kit

Recombinant
SimpleStep
What is this?

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(4 Publications)

Human Cardiac Troponin T ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cardiac Troponin T with a sensitivity of 68.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TnTc, Cardiac muscle troponin T, cTnT, TNNT2

3 Images

ELISA - Human Cardiac Troponin T ELISA Kit (AB223860)

ELISA

Supplier Data

ELISA - Human Cardiac Troponin T ELISA Kit (AB223860)

sELISA

Supplier Data

Sandwich ELISA - Human Cardiac Troponin T ELISA Kit (AB223860)

Interpolated concentrations of native Cardiac Troponin T in human heart tissue extract based on a 100 μg/mL extract load.

The concentrations of Cardiac Troponin T were measured in duplicate and interpolated from the Cardiac Troponin T standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin T concentration was determined to be 378.8 pg/mL in heart tissue extract

sELISA

Supplier Data

Sandwich ELISA - Human Cardiac Troponin T ELISA Kit (AB223860)

Example of human Cardiac Troponin T standard curve in Sample Diluent NS.

The Cardiac Troponin T standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 68.2 pg/mL

Range

195.3 - 12500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Cardiac Troponin T ELISA Kit ab223860 is a rapid single-wash 90-min Sandwich ELISA to measure Human Cardiac Troponin T in cell culture media, serum. This SimpleStep sensitivity is 68.2 pg/mL.

How the assay works

Human Cardiac Troponin T SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Cardiac Troponin T ELISA Kit ab223860 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Cardiac Troponin T antibody [EPR20266-143] - BSA and Azide free (Capture) ab252655
- Anti-Cardiac Troponin T antibody [EPR20266-143-6] - BSA and Azide free (Detector) ab252655

Cardiac Troponin T is one of the three tropomyosin binding subunit that makes up the troponin complex with TnI and TnC being the other two subunits. The troponin complex is essential to the contraction of skeletal and heart muscles. Troponin T specifically binds to tropomyosin and helps position it on actin. Studies have shown elevated levels of troponin T after myocardial infarction and significantly associated with the incidence of heart failure and cardiovascular death.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.96" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.82" } ]

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Recovery

[ { "sample": "Serum", "range": "83 - 93 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "80 - 89 %", "average": "= 84" }, { "sample": "Tissue Extracts", "range": "76 - 88 %", "average": "= 83" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab223860-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB223860-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB223860-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB223860-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB223860-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB223860-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB223860-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cardiac Troponin T Capture Antibody", "number":"AB223860-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cardiac Troponin T Lyophilized Recombinant Protein", "number":"AB223860-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cardiac Troponin T Detector Antibody", "number":"AB223860-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB223860-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB223860-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNT2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401415 PubMed38965824

2024

Modeling the Effects of Protracted Cosmic Radiation in a Human Organ-on-Chip Platform.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Naveed Tavakol,Trevor R Nash,Youngbin Kim,Pamela L Graney,Martin Liberman,Sharon Fleischer,Roberta I Lock,Aaron O'Donnell,Leah Andrews,Derek Ning,Keith Yeager,Andrew Harken,Naresh Deoli,Sally A Amundson,Guy Garty,Kam W Leong,David J Brenner,Gordana Vunjak-Novakovic

Disease models & mechanisms 17: PubMed38050701

2024

Physiological stress improves stem cell modeling of dystrophic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Dominic E Fullenkamp,Alexander B Willis,Jodi L Curtin,Ansel P Amaral,Kyle T Dittloff,Sloane I Harris,Ivana A Chychula,Cory W Holgren,Paul W Burridge,Brenda Russell,Alexis R Demonbreun,Elizabeth M McNally

Molecules (Basel, Switzerland) 28: PubMed37513325

2023

Phenolics Extracted from Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Urooj Umar,Sibtain Ahmed,Asra Iftikhar,Maryam Iftikhar,Wafa Majeed,Atika Liaqat,Sana Shahzad,Mateen Abbas,Tahir Mehmood,Farooq Anwar

PLoS neglected tropical diseases 16:e0010864 PubMed36228040

2022

Clinical and prognostic relevance of sST2 in adults with dengue-associated cardiac impairment and severe dengue.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Teo,Po Ying Chia,Gaurav Kumar Ramireddi,Sebastian Kah Ming Khoo,Tsin Wen Yeo

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Cardiac Troponin T ELISA Kit

ELISA - Human Cardiac Troponin T ELISA Kit (AB223860)

Sandwich ELISA - Human Cardiac Troponin T ELISA Kit (AB223860)

Sandwich ELISA - Human Cardiac Troponin T ELISA Kit (AB223860)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (4)

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Product promise