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AB99996

Human Cardiotrophin 1 ELISA Kit (CTF1)

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Human Cardiotrophin 1 ELISA Kit (CTF1) is a Sandwich ELISA for the measurement of Human Cardiotrophin 1 (CTF1) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Cardiotrophin-1, CT-1, CTF1

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ELISA - Human Cardiotrophin 1 ELISA Kit (CTF1) (AB99996)
  • ELISA

Lab

ELISA - Human Cardiotrophin 1 ELISA Kit (CTF1) (AB99996)

Standard curve with background signal subtracted (duplicates; +/- SD).

Sandwich ELISA - Human Cardiotrophin 1 ELISA Kit (CTF1) (AB99996)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cardiotrophin 1 ELISA Kit (CTF1) (AB99996)

Representative Standard Curve using ab99996.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 20 pg/mL

Range

16.46 - 12000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Cardiotrophin 1 (CTF1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cardiotrophin 1 in plasma and cell culture supernatants. (Human Cardiotrophin 1 concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for Human Cardiotrophin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Cardiotrophin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Cardiotrophin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cardiotrophin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect the endogenous Human Cardiotrophin 1 in normal serum with ab99996, only in serum spiked with Human Cardiotrophin 1.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "76 - 112 %", "average": "= 98.2" }, { "sample": "Plasma", "range": "70 - 134 %", "average": "= 101.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99996-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99996-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99996-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Cardiotrophin 1 Human Standard (lyophilized)", "number":"AB99996-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Cardiotrophin 1", "number":"AB99996-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cardiotrophin 1 Microplate (12 x 8 wells)", "number":"AB99996-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB99996-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99996-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB99996-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
See full target information CTF1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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