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AB119508

Human Caspase-9 ELISA Kit

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(6 Publications)

Human Caspase-9 ELISA Kit is a Sandwich ELISA for the measurement of Human Caspase-9 in Human in Cell Culture Media, Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

1 Images
Sandwich ELISA - Human Caspase-9 ELISA Kit (AB119508)
  • sELISA

Supplier Data

Sandwich ELISA - Human Caspase-9 ELISA Kit (AB119508)

Representative Standard Curve using ab119508.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.4 ng/mL

Range

1.6 - 100 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Caspase-9 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Caspase-9 concentrations in cell lysate, cell culture supernatant and serum.

Caspase-9 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Caspase-9 detection antibody and the microplate is then incubated at room temperature. Following washing a HRP conjugated anti-rabbit-IgG antibody is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Caspase-9 amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119508-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"20X Assay Buffer Concentrate", "number":"AB119508-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB119508-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate coated with monoclonal antibody to Human Caspase-9 (12 x 8 wells)", "number":"AB119508-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Lysis buffer", "number":"AB119508-CMP07", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Films", "number":"AB119508-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent", "number":"AB119508-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Caspase-9 Standard", "number":"AB119508-CMP06", "productcode":"" }, { "size":"1 x 70 µL", "name":"Polyclonal anti-Human Caspase-9 Detection Antibody (rabbit)", "number":"AB119508-CMP03", "productcode":"" }, { "size":"1 x 10 µL", "name":"Anti-rabbit-IgG HRP conjugated antibody", "number":"AB119508-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution (1M Phosphoric acid)", "number":"AB119508-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB119508-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758105, PubMed : 36758106).. Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.
See full target information CASP9
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 24:2246198 PubMed37773732

2023

inhibits the growth and metastasis of liver cancer by downregulating miR-9-5p and upregulating PDK4.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Si,Liyin Huang,Ting Liang,Ping Huang,Hongyu Zhang,Mingmin Zhang,Xiaoling Zhou

ACS omega 7:46629-46639 PubMed36570308

2022

Comparative Anticancer Potentials of Taxifolin and Quercetin Methylated Derivatives against HCT-116 Cell Lines: Effects of -Methylation on Taxifolin and Quercetin as Preliminary Natural Leads.

Applications

Unspecified application

Species

Unspecified reactive species

Hamdoon A Mohammed,Suliman A Almahmoud,El-Sayed M El-Ghaly,Firdos Alam Khan,Abdul-Hamid Emwas,Mariusz Jaremko,Fatimah Almulhim,Riaz A Khan,Ehab A Ragab

Neuroimmunomodulation 28:266-275 PubMed33951651

2021

Activation of Insulin-Like Growth Factor-2 Ameliorates Retinal Cell Damage and Exerts Protection in in vitro Model of Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yantao Zhao,Zhaohui Xiong,Yuping Chen,Guoqiang Wang,Yan Zhao

Journal of Cancer 12:3393-3406 PubMed33976749

2021

ZFPM2-AS1 transcriptionally mediated by STAT1 regulates thyroid cancer cell growth, migration and invasion via miR-515-5p/TUSC3.

Applications

Unspecified application

Species

Unspecified reactive species

Ruizhen Ren,Yuanna Du,Xing Niu,Rukun Zang

Medicine 99:e20922 PubMed32590803

2020

Apoptosis in cerebrospinal fluid as outcome predictors in severe traumatic brain injury: An observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqing Jiang,Peng Jin,Wenfeng Wei,Wei Jiang

Molecular medicine reports 13:2689-95 PubMed26848042

2016

Poly (I:C) transfection induces mitochondrial-mediated apoptosis in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Chen,Dong-Liang Wang,Yu-Ling Liu
View all publications
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websiteProtocolBooklet
en
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Product promise

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