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AB309398

Human Caspr2/CNTNAP2 ELISA Kit

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Human Caspr2/CNTNAP2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Caspr2/CNTNAP2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CASPR2, KIAA0868, CNTNAP2, Contactin-associated protein-like 2, Cell recognition molecule Caspr2

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Sandwich ELISA - Human Caspr2/CNTNAP2 ELISA Kit (AB309398)
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Supplier Data

Sandwich ELISA - Human Caspr2/CNTNAP2 ELISA Kit (AB309398)

Example of standard curve using ab309398. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 26 pg/mL

Range

26 - 6000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Caspr2/CNTNAP2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Caspr2/CNTNAP2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Caspr2/CNTNAP2 coated on a 96-well plate.
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Recovery

[ { "sample": "Plasma", "range": "96 - 113 %", "average": "= 105.2" }, { "sample": "Serum", "range": "108 - 112 %", "average": "= 110.9" }, { "sample": "Cell culture media", "range": "95 - 109 %", "average": "= 104.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309398-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human Caspr2 Detection Antibody", "number":"AB309398-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Caspr2 Antibody-coated ELISA Plate", "number":"AB309398-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309398-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309398-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Caspr2 Protein Standard", "number":"AB309398-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB309398-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309398-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309398-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB309398-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caspr2 also known as CNTNAP2 is a member of the neurexin family with a molecular mass of approximately 133 kDa. This protein extensively expresses in the nervous system and particularly locates on the axonal membrane. Caspr2 functions as a cell adhesion molecule playing a role in facilitating interactions between neurons and glial cells. Additionally Caspr2 participates in the clustering of ion channels at the nodes of Ranvier which is important for rapid action potential propagation along myelinated axons.
Biological function summary

This protein is important in maintaining the integrity of the neuronal membrane. Caspr2 works as part of a complex associating with contactin proteins to stabilize neuronal networks. It is involved in synaptic plasticity and potentially influences neuronal connectivity and communication. Through its adhesive functions Caspr2 contributes to structural frameworks that ensure proper signal transmission in the neural circuits.

Pathways

Caspr2 participates in pathways responsible for neuronal excitability and membrane potential regulation. It plays an important role within the voltage-gated potassium channel complex. Caspr2 also connects to proteins like TAG-1 forming a vital part of the complex involved in the assembly of potassium channels and ensuring efficient nerve impulse conduction. Its interaction within these pathways impacts synaptic function and cognitive processes by modulating neuronal excitability.

Caspr2 has links to epilepsy and autism spectrum disorders where disruptions in its function can lead to pathogenic outcomes. Alterations in Caspr2 expression or structure may result in impaired neuron-glial interactions contributing to neurological symptoms. It relates to contactin-associated proteins in these contexts potentially affecting the development and maintenance of neuronal structures. Understanding Caspr2’s involvement in these diseases helps in developing therapeutic strategies and antibodies such as anti-Caspr2 antibodies to modulate its activity and effects.
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Product protocols

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Target data

Required for gap junction formation (Probable). Required, with CNTNAP1, for radial and longitudinal organization of myelinated axons. Plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Demarcates the juxtaparanodal region of the axo-glial junction.
See full target information CNTNAP2
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websiteProtocolBooklet
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Product promise

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