Human Cathepsin B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cathepsin B with a sensitivity of 23.44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Journal of sport and health science 12:175-1852022The effects of a 20-week exercise program on blood-circulating biomarkers related to brain health in overweight or obese children: The ActiveBrains project.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Rodriguez-Ayllon,Abel Plaza-Florido,Andrea Mendez-Gutierrez,Signe Altmäe,Patricio Solis-Urra,Concepción M Aguilera,Andrés Catena,Francisco B Ortega,Irene Esteban-CornejoPubMed 36529369
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 109.38 - 7000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 23.44 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed:12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed:3972105).
Alternative names
CPSB, CTSB, Cathepsin B, APP secretase, Cathepsin B1, APPS
What's included?
Recommended products
Human Cathepsin B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cathepsin B with a sensitivity of 23.44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 109.38 - 7000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 23.44 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 4.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 100 | Range 87 - 106 % |
Sample type Serum | Average % = 98 | Range 95 - 99 % |
Sample type EDTA Plasma | Average % = 114 | Range 110 - 122 % |
Sample type Saliva | Average % = 108 | Range 95 - 108 % |
Sample type Urine | Average % = 91 | Range 84 - 100 % |
Sample type Heparin Plasma | Average % = 103 | Range 92 - 113 % |
Sample type Citrate plasma | Average % = 93 | Range 88 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Cathepsin B ELISA kit (ab272205) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin B protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cathepsin B with 41.8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cathepsin B is a part of a family of cysteine lysosomal proteases. Cathepsin B is synthesized as a preproenzyme in the endoplasmic reticulum and activated after transportation to the Golgi apparatus. Cathepsin B is overexpressed in invasive and metastatic cancers and is such a potential biomarker for a variety of cancers. Double knockout of cathepsin B and cathepsin L in mice is lethal due to their role in the central nervous system.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.
- Biological function summary
Cathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.
- Pathways
Cathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.
- Associated diseases and disorders
Cathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.
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5 product images
Sandwich ELISA - Human Cathepsin B ELISA Kit (ab272205)
Human Cathespin B competitor curve comparison.
Standard Curve comparison between Human Cathepsin B SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Cathepsin B ELISA Kit (ab272205)
Example of human Cathepsin B standard curve in Sample Diluent NS.
The Cathepsin B standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Cathepsin B ELISA Kit (ab272205)
Interpolated concentrations of native Cathepsin B in human serum and plasma samples.
The concentrations of Cathepsin B were measured in duplicates, interpolated from the Cathepsin B standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 8%, plasma (citrate) 10%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 54.38 ng/mL in serum, 56.17 ng/mL in plasma (citrate), 55.98 ng/ml in plasma (heparin), 55.79 ng/ml in plasma (EDTA).
Sandwich ELISA - Human Cathepsin B ELISA Kit (ab272205)
Interpolated concentrations of native Cathepsin B in treated human PBMC cell culture supernatant and male saliva samples.
PBMC supernatant samples were treated with 1.5% PHA-M for 46 hours. The concentrations of Cathepsin B were measured in duplicates, interpolated from the Cathepsin B standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 4% and saliva 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 103.07 ng/mL in treated PBMC supernatant and 7.00 ng/mL in male saliva.
Sandwich ELISA - Human Cathepsin B ELISA Kit (ab272205)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 98.94 ng/mL with a range of 54.68 – 219.68 ng/mL.
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