JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272205

Human Cathepsin B ELISA Kit

Be the first to review this product! Submit a review

|

(2 Publications)

Human Cathepsin B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cathepsin B with a sensitivity of 23.44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CPSB, CTSB, Cathepsin B, APP secretase, Cathepsin B1, APPS

6 Images
ELISA - Human Cathepsin B ELISA Kit (AB272205)
  • ELISA

Supplier Data

ELISA - Human Cathepsin B ELISA Kit (AB272205)
Sandwich ELISA - Human Cathepsin B ELISA Kit (AB272205)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin B ELISA Kit (AB272205)

Interpolated concentrations of native Cathepsin B in treated human PBMC cell culture supernatant and male saliva samples.

PBMC supernatant samples were treated with 1.5% PHA-M for 46 hours. The concentrations of Cathepsin B were measured in duplicates, interpolated from the Cathepsin B standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 4% and saliva 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 103.07 ng/mL in treated PBMC supernatant and 7.00 ng/mL in male saliva.

Sandwich ELISA - Human Cathepsin B ELISA Kit (AB272205)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin B ELISA Kit (AB272205)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 98.94 ng/mL with a range of 54.68 – 219.68 ng/mL.

Sandwich ELISA - Human Cathepsin B ELISA Kit (AB272205)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin B ELISA Kit (AB272205)

Human Cathespin B competitor curve comparison.

Standard Curve comparison between Human Cathepsin B SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Cathepsin B ELISA Kit (AB272205)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin B ELISA Kit (AB272205)

Interpolated concentrations of native Cathepsin B in human serum and plasma samples.

The concentrations of Cathepsin B were measured in duplicates, interpolated from the Cathepsin B standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 8%, plasma (citrate) 10%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 54.38 ng/mL in serum, 56.17 ng/mL in plasma (citrate), 55.98 ng/ml in plasma (heparin), 55.79 ng/ml in plasma (EDTA).

Sandwich ELISA - Human Cathepsin B ELISA Kit (AB272205)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin B ELISA Kit (AB272205)

Example of human Cathepsin B standard curve in Sample Diluent NS.

The Cathepsin B standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 23.44 pg/mL

Range

109.38 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Cathepsin B ELISA Kit ab272205 is a rapid single-wash 90-min Sandwich ELISA to measure Human Cathepsin B in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum, urine. This SimpleStep sensitivity is 23.44 pg/mL.

How the assay works

Human Cathepsin B SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Cathepsin B ELISA Kit ab272205 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Cathepsin B antibody [EPR22982-114] - BSA and Azide free (Capture) ab272409
- Anti-Cathepsin B antibody [EPR22982-124] - BSA and Azide free (Detector) ab272409

Cathepsin B is a part of a family of cysteine lysosomal proteases. Cathepsin B is synthesized as a preproenzyme in the endoplasmic reticulum and activated after transportation to the Golgi apparatus. Cathepsin B is overexpressed in invasive and metastatic cancers and is such a potential biomarker for a variety of cancers. Double knockout of cathepsin B and cathepsin L in mice is lethal due to their role in the central nervous system.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 106 %", "average": "= 100" }, { "sample": "Serum", "range": "95 - 99 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "110 - 122 %", "average": "= 114" }, { "sample": "Saliva", "range": "95 - 108 %", "average": "= 108" }, { "sample": "Urine", "range": "84 - 100 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "92 - 113 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "88 - 101 %", "average": "= 93" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272205-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272205-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272205-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272205-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272205-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272205-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cathepsin B Capture Antibody", "number":"AB272205-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cathepsin B Lyophilized Recombinant Protein", "number":"AB272205-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB272205-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272205-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cathepsin B Detector Antibody", "number":"AB272205-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.
Biological function summary

Cathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.

Pathways

Cathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.

Cathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.
style
left-column

Product protocols

style
left-column

Target data

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed : 12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed : 12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed : 3972105).
See full target information CTSB
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 194:493-499 PubMed38819461

2024

Influence of stimulation frequency on brain-derived neurotrophic factor and cathepsin-B production in healthy young adults.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichi Nishikawa,Hiroyuki Sakaguchi,Tatsuya Takada,Noriaki Maeda,Allison Hyngstrom

Journal of sport and health science 12:175-185 PubMed36529369

2022

The effects of a 20-week exercise program on blood-circulating biomarkers related to brain health in overweight or obese children: The ActiveBrains project.

Applications

Unspecified application

Species

Unspecified reactive species

María Rodriguez-Ayllon,Abel Plaza-Florido,Andrea Mendez-Gutierrez,Signe Altmäe,Patricio Solis-Urra,Concepción M Aguilera,Andrés Catena,Francisco B Ortega,Irene Esteban-Cornejo
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB119586

Human Cathepsin D ELISA Kit

Sandwich ELISA - Human Cathepsin D ELISA Kit (AB119586)

  • 0
  • 0
Assay Kits

AB155427

Human Cathepsin S ELISA Kit (CTSS)

Sandwich ELISA - Human Cathepsin S ELISA Kit (CTSS) (AB155427)

  • 0
  • 0
Assay Kits

AB65300

Cathepsin B Activity Assay Kit (Fluorometric)

Functional Studies - Cathepsin B Activity Assay Kit (Fluorometric) (AB65300)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com